PTEN对H_2O_2诱导的牙周膜细胞增殖凋亡的影响  

Effects of PTEN on Proliferation and Apoptosis of Periodontal Ligament Cells Induced by Hydrogen Peroxide.

作　　者：赵媛[1] 王姝[2] 韶波 高颖[1] 

机构地区：[1]郑州大学附属郑州中心医院口腔颌面外科,河南郑州450007 [2]内蒙古医科大学附属医院口腔颌面外科,内蒙古呼和浩特010000 [3]内蒙古自治区人民医院口腔科,内蒙古呼和浩特010000

出　　处：《口腔医学研究》2017年第8期889-892,共4页Journal of Oral Science Research

基　　金：河南省医学科技攻关计划项目(编号:201303100);内蒙古医科大学青年创新基金项目(编号:YKD2015QNCX042)

摘　　要：目的:探讨第10号染色体同源缺失性磷酸酶-张力蛋白(PTEN)对过氧化氢(H2O2)诱导的牙周膜细胞增殖凋亡的影响。方法:分离培养人牙周膜细胞,转染PTEN小干扰RNA、小干扰RNA阴性对照,H2O2处理,Western blot检测PTEN表达水平。结果:人牙周膜细胞转染PTEN小干扰RNA后的细胞中PTEN表达受到抑制。结论:干扰PTEN表达能够拮抗H2O2诱导的人牙周膜细胞凋亡及人牙周膜细胞增殖抑制作用。Objective：To investigate the effects of PTEN on proliferation and apoptosis of periodontal ligament cells induced by hydrogen peroxide.Methods：Human periodontal ligament cells were isolated and cultured in vitro,and then transfected with PTEN small interfering RNA or small interfering RNA negative control.After H2O2 treatment,PTEN expression was detected in cells by Western blot.Results：PTEN expression was inhibited in human periodontal ligament cells transfected with PTEN small interfering RNA.Conclusion：Interference of PTEN expression could inhibit the apoptosis and the proliferation of human periodontal ligament cells induced by H2O2.

关 键 词：牙周膜细胞 增殖 凋亡 第10号染色体同源缺失性磷酸酶-张力蛋白 过氧化氢 

分 类 号：R781[医药卫生—口腔医学]