阿霉素磁性介孔氧化硅纳米颗粒对口腔鳞状细胞癌细胞的体外实验研究  

Therapy of Oral Squamous Cell Carcinoma using Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles in Vitro

作　　者：薛本哲 李善昌[1] 杨绍威 董波[3] 曲学延[1] 

机构地区：[1]佳木斯大学附属第二医院口腔医院颌面外科二科,佳木斯大学,黑龙江佳木斯154007 [2]嘉兴市第二医院口腔科,浙江嘉兴314000 [3]佳木斯大学附属第二医院口腔医院牙体牙髓科一科,黑龙江佳木斯154007

出　　处：《口腔医学研究》2017年第8期893-897,共5页Journal of Oral Science Research

基　　金：佳木斯大学研究生科技创新项目(编号:LZZ2015-021);黑龙江省教育厅科学技术研究项目(编号:12521553)

摘　　要：目的:探讨阿霉素磁性介孔氧化硅纳米颗粒(Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles,DOX@M-MSNs)对口腔鳞状细胞癌细胞的体外靶向杀伤作用。方法:采用甲基噻唑基四唑(MTT)法,流式细胞仪及激光共聚焦显微镜检测DOX@M-MSNs对CAL-27的体外靶向抗肿瘤效果。结果:单纯磁性介孔氧化硅纳米颗粒表现出非常好的生物稳定性以及生物相容性,尽管浓度为100 mg/L时,细胞存活率仍超过80%。当浓度值有所提升及作用时间延长后,相较于单纯阿霉素原料组,DOX@M-MSNs对于肿瘤细胞的增殖抑制作用明显增强,差异有统计学意义(P<0.05)。流式细胞仪检测结果显示DOX@M-MSNs促进肿瘤细胞的凋亡率高于单纯阿霉素,随着药物作用时间延长,其对肿瘤细胞的抑制效果明显增强,凋亡率增高。激光共聚焦显微镜检测结果显示通过外加磁场,磁性区域的DOX@M-MSN释放的DOX于肿瘤细胞的聚集优于非磁性区域。结论:DOX@M-MSNs纳米颗粒能够选择性进入肿瘤细胞内,从而使得阿霉素对肿瘤细胞的毒性有所增强,进而使得其杀伤肿瘤细胞的效果显著提高,外加磁场可以显著地提升输送DOX@M-MSNs进到肿瘤细胞内的效率,进而提高肿瘤抑制效果。Objective：To investigate the targeting effect of doxorubicin-loading mesoporous magnetic silia nanoparticles（DOX@ M-MSNs）on oral squamous carcinomas cells in vitro.Methods：Methyl thiazolyl tetrazolium（MTT）,flow cytometry,and laser scanning confocal microscope were used to detect the targeted anti-tumor activity of DOX@M-MSNs against CAL-27.Results：The blank mesoporous magnetic silia nanoparticles showed excellent biocompatibility with cell viability〉80% at the maximum tested concentration.With increase of concentration and the going of time,the inhibition of tumor cell proliferation from DOX@M-MSNs was superior to that from free DOX（P〈0.05）.Flow cytometry showed that DOX@M-MSNs induced higher necrotic rates than free DOX,and with the elapse of time,the necrosis rate increased.Laser scanning confocal microscope showed that themagnetic area of DOX @ M-MSNs in gathered was better than that of non-magnetic regions of the tumor cells through external magnetic field,compared with non-magnetic area,the magnetic area showed a higher cell death.Conclusion：DOX@M-MSNs can selectively recognize oral squamous carcinomas cells,enhance to deliver DOX into the cells,and promote its effect in killing oral squamous carcino-mas cells.By applying the magnetic field,the drug delivery efficiency of DOX@M-MSNs can be increased.

关 键 词：阿霉素 介孔氧化硅纳米颗粒 外部磁场 靶向作用 肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]