HPLC法同时测定民族药材青阳参中6种酚类成分的含量  

Simultaneous determination of 6 phenols in ethnic medicine Radix Cynanchi Otophylli by HPLC

作　　者：昝珂[1] 黄莉莉 刘杰[1] 过立农[1] 张雯洁 郑健[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]广西壮族自治区食品药品检验所,南宁530021 [3]云南省食品药品监督检验研究院,昆明650000

出　　处：《药物分析杂志》2017年第8期1447-1452,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：国家食药总局药化注册司专项"12种民族药材检验方法示范性研究";十二五国家科技重大专项(2014ZX09304307-002)

摘　　要：目的:建立高效液相色谱法同时测定民族药材青阳参中对羟基苯甲酸、香草酸、对羟基苯乙酮、2,5-二羟基苯乙酮、白首乌二苯酮和2,4-二羟基苯乙酮的含量。方法:采用HSS T_3C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.05%甲酸水溶液(20∶80)为流动相,流速1 m L·min^(-1),检测波长为260 nm,柱温30℃,进样量10μL。结果:对羟基苯甲酸、香草酸、对羟基苯乙酮、2,5-二羟基苯乙酮、白首乌二苯酮和2,4-二羟基苯乙酮质量浓度分别在0.082~4.084μg·m L^(-1)(r=0.999 6)、0.080~4.024μg·m L^(-1)(r=0.999 8)、0.158~7.888μg·m L^(-1)(r=0.999 7)、0.078~3.900μg·m L^(-1)(r=0.999 7)、0.308~15.41μg·m L^(-1)(r=0.999 6)和0.077~3.872μg·m L^(-1)(r=0.999 8)范围内线性关系良好,平均加样回收率、精密度、重现性和稳定性均符合有关规定。样品中6个酚类成分的含量分别为对羟基苯甲酸0.019~0.133 mg·g^(-1),香草酸0.021~0.140 mg·g^(-1),对羟基苯乙酮0.068~0.300 mg·g^(-1),2,5-二羟基苯乙酮0.029~0.383 mg·g^(-1),白首乌二苯酮0.220~1.507 mg·g^(-1),2,4-二羟基苯乙酮0.034~0.369 mg·g^(-1)。结论:本法可用于青阳参中6个酚类成分的含量测定。Objective： To establish an HPLC method for the simultaneous determination of 6 phenols, including p-hydroxybenzoic acid, vanillic acid, p-hydroxyacetophenone, 2, 4-dihydroxyacetophenone,baishouwubenzophenone and 2, 5-dihydroxyacetophenone in ethnic medicine Radix Cynanchi Otophylli. Methods： HPLC analysis was performed on a HSS T3 C18 （250 mm × 4.6 mm, 5 μm ）column. The mobile phase was acetonitrile-0.05% formic acid solution （ 20 ： 80 ） at the flow rate of 1 mL·min^-1 and the detection wavelength was 260 nm. The column temperature was 30 ℃ and the injection volume was 10 μL. Results： The standard curves revealed a good linear relationship in the ranges of 0.082-4.084 μg·mL^-1 （ r=0.999 6 ） for p-hydroxybenzoic acid, 0.080-4.024 μg·mL^-1 （ r=0.999 8 ）for vanillic acid, 0.158-7.888 μg·mL^-1（ r=0.999 7 ） for p-hydroxyaeetophenone, 0.078-3.900 μg·mL^-1（ r=0.999 7 ） for 2, 5-dihydroxyacetophenone, 0.308- 15.41μg·mL^-1（ r=0.999 6 ）for baishouwubenzophenone and 0.077-3.872 μg·mL^-1（ r=0.999 8 ）for 2, 4-dihydroxyaeetophenone, respectively. The contents of 6 phenols were 0.019-0.133 mg·g^-1 for p-hydroxybenzoic acid, 0.021-0.140 mg·g^-1 for vanillie acid, 0.068-0.300 mg·g^-1 for p-hydroxyacetophenone, 0.029-0.383 mg·g^-1 for 2, 5-dihydroxyacetophenone, 0.220-1.507 mg·g^-1 for baishouwubenzophenone and 0.034-0.369mg·g^-1 for 2,4-dihydroxyacetophenone, respectively. Conclusion： The developed method is suitable for the quantitative analysis of six phenols in Radix Cynanchi Otophylli.

关 键 词：青阳参 酚类成分 对羟基苯甲酸 香草酸 对羟基苯乙酮 二羟基苯乙酮 白首乌二苯酮 天然药物多组分测定 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]