胃癌多药耐药细胞中FOXC1的表达及其意义  

The expression and significance of FOXC1 in multidrug-resistance cells of gastric cancer

作　　者：安艳新[1] 孙力勇[1] 王菁[2] 

机构地区：[1]原济南军区总医院普外科,山东济南250031 [2]原济南军区总医院口腔科,山东济南250031

出　　处：《实用医药杂志》2017年第8期687-689,共3页Practical Journal of Medicine & Pharmacy

基　　金：国家自然科学基金(81602615)

摘　　要：目的探讨FOXC1在胃癌耐药细胞中的表达情况及对胃癌细胞药物敏感性的影响。方法采用q RT-PCR、Western Blot法检测胃癌多药耐药细胞SGC7901/ADR、SGC7901/VCR及亲本细胞SGC7901中FOXC1的表达;应用si RNA下调胃癌多药耐药细胞SGC7901/ADR、SGC7901/VCR中FOXC1的表达后以MTT法检测上述细胞IC50值的变化。结果 FOXC1在胃癌多药耐药细胞SGC7901/ADR、SGC7901/VCR表达显著高于亲本细胞SGC7901,当下调其表达后,SGC7901/ADR、SGC7901/VCR对多种化疗药物的敏感性显著升高。结论 FOXC1参与胃癌多药耐药过程,但其相关分子机制有待进一步深入研究。Objective To investigate the expression of FOXCI in muhidrug-resistance ceils of gastric cancer and its effect on chemosensitivity of gastric cancer cells. Methods qRT-PCR and Western blot were used to detect the expression of FOXC1 in the cells of SGC7901/ADR,SGC7901/VCR and parent cells of SGC7901; the expression of FOXC1 in SGC7901/ADR and SGC7901/VCR was down-regulated by siRNA,the IC50 values of these cells were detected by MTY assay. Results The expression of FOXC1 in SGC7901/ADR and SGC7901/VCR was siguifieantly higher than that in SGC7901. After the expression of FOXC1 was down-regulated,the sensitivity of SGC7901/ADR and SGC7901/VCR to various chemotherapeutic drugs was significantly increased. Conclusion FOXC1 is involved in muhidrug-resistanee process of gastric cancer,but its molecular mechanism remains to be further studied.

关 键 词：FOXC1 胃癌 多药耐药性 

分 类 号：R735.2[医药卫生—肿瘤] R979.1[医药卫生—临床医学]