傅立叶变换红外光谱技术对6种致病性大肠埃希氏菌快速分型的研究  

Rapid classification for 6 species of pathogenic Escherichia coli by Fourier transform infrared spectroscopy

作　　者：张慧敏 李兆杰 魏玮 杨丽君 高飞 鞠玲燕 李正义[2] 徐成钢 

机构地区：[1]威海出入境检验检疫局,威海264205 [2]山东出入境检验检疫局,青岛266002

出　　处：《食品安全质量检测学报》2017年第1期216-222,共7页Journal of Food Safety and Quality

基　　金：国家质量监督检验检疫总局科研项目(2015IK204;2015IK203)~~

摘　　要：目的建立肠致病性大肠埃希氏菌(Enteropathogenic E.coli,EPEC)、肠产毒性大肠埃希氏菌(Enterotoxigenic E.coli,ETEC)、肠侵袭性大肠埃希氏菌(Enteroinvasive E.coli,EIEC)、肠黏附性大肠埃希氏菌(Enteroadhesive E.coli,EAEC)、肠出血性大肠埃希氏菌(Enterohmorrhagic E.coli,EHEC)、弥散粘附性大肠埃希氏菌(Diffusely adherent E.coli,DAEC)6种致病性大肠埃希氏菌的傅立叶变换红外光谱(Fourier transform infrared spectroscopy,FT-IR)数据库及FT-IR分型方法。方法应用FT-IR技术对6种致病性大肠埃希氏菌进行指纹图谱数据采集并对其进行基线校正、归一化等处理,应用化学计量学方法对光谱数据进行分析。结果本研究建立了6种致病性大肠埃希氏菌的FT-IR光谱数据库,实现了对6种可疑目标大肠埃希氏菌鉴定;建立了主成分分析(principal component analysis,PCA)和分级聚类分析(hierarchical cluster analysis,HCA)2种模型,成功实现了对6种致病性大肠埃希氏菌的快速分型。结论 FT-IR分析方法快速、简便、易操作,结果重现性好,是一种鉴定6种致病性大肠埃希氏菌的有效方法。Objective To establish Fourier transform infrared spectroscopy（FT-IR） database and a FT-IR classification method for 6 species of Escherichia coli（E. coli）, including Enteropathogenic E.coli（EPEC）, Enterotoxigenic E.coli（ETEC）, Enteroinvasive E.coli（EIEC）, Enteroadhesive E.coli（EAEC）, Enterohmorrhagic E.coli（EHEC） and Diffusely adherent E.coli（DAEC）. Methods FT-IR fingerprint absorption spectra of 6 species of E. coli were collected, treated by baseline correction and vector normalization and so on, and then analyzed by chemometric methods. Results A standard FT-IR spectral library including 6 species of E. coli was created and could be used to identify the target E. coli; 2 cluster models of principal component analysis（PCA） and hierarchical cluster analysis（HCA） were developed and could classify the 6 species of E. coli successfully. Conclusion As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy is an effective tool to differentiate and identify 6 species of E. coli.

关 键 词：傅立叶变换红外光谱 致病性大肠埃希氏菌 主成分分析 分级聚类分析 分型 

分 类 号：R446.5[医药卫生—诊断学] O657.33[医药卫生—临床医学] TS207.4[理学—分析化学]