植物病毒检测及脱毒方法的研究进展  

Research Advances on Plant Virus Detection and Virus-elimination Methods

作　　者：迟惠荣 毛碧增[1] 

机构地区：[1]浙江大学农业与生物技术学院,杭州310058

出　　处：《生物技术通报》2017年第8期26-33,共8页Biotechnology Bulletin

基　　金：浙江省农业中药材新品种选育重大科技专项(2016C02058)

摘　　要：病毒是人们所知的自然界最小的生物之一,其形态大小要借助电镜放大到几万甚至几十万倍才能观察得到。病毒具有严格的寄生性,必须在宿主细胞内才能生长繁殖。植物病毒是病毒科的一个重要组成部分,这一类病原物所引起的病害正逐年加重,影响了植物的生长和发育,降低了产量和品质。因病毒种类多,危害机制复杂,所以病毒病的防治也存在一定的困难。阐述了植物病毒的检测方法,详述了脱毒的技术方法及其近几年的应用情况,讨论了在脱毒过程中要注意的问题,并对今后的研究方向进行展望,以期为相关研究提供一些参考和理论依据。Virus is known as one of the smallest creatures in nature,its morphology and size can only be observed and identified byelectron microscopy enlarging it by tens of thousands or even hundreds of thousands of times. The virus is strictly parasitic so that its growth andreproduction depend on the host cell. Plant viruses are an important part of the virus family. The plant virus disease,which affects the growthand development of plants and decreases the yield and quality,is increasingly serious year by year. Due to the numerous varieties of viruses andcomplex damage mechanisms,it is difficult to prevent and control the viral diseases. This article reviews the major methods of detecting plantvirus as well as the technologies of virus-elimination and its application in recent years. Further,the article discusses the issues which need tobe paid attention to in the virus-elimination. Finally,the article forecasts the future research direction for providing references and theoreticalbasis for further study.

关 键 词：植物病毒 检测技术 脱毒方法 

分 类 号：S432.41[农业科学—植物病理学]