沉香醇提物对四氯化碳致小鼠急性肝损伤的保护作用  

Protective Effects of Alcohol Extract of Agarwood on Acute Liver Injury Induced by Carbon Tetrachloride in Mice

作　　者：王灿红 王帅[2] 彭德乾[3] 刘洋洋[1] 郭鹏[1,2] 魏建和[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所(海南省南药资源保护与开发重点实验室国家中医药管理局沉香可持续利用重点研究室),海南海口570311 [2]中国医学科学院北京协和医学院药用植物研究所(中草药物质基础与资源利用教育部重点实验室濒危药材繁育国家工程实验室),北京100193 [3]海南医学院药学院,海南海口571199

出　　处：《中国现代中药》2017年第8期1091-1096,共6页Modern Chinese Medicine

基　　金：海南省重大科技计划项目(ZDKJ2016004);海南省自然科学基金(817290);国家自然科学基金资助项目(81673545);中组部"万人计划"(99950534)

摘　　要：目的:探讨沉香醇提物对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:采用CCl4腹腔注射制备小鼠急性肝损伤模型,给药处理后计算肝脏指数;检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;肝组织匀浆液中髓过氧化物酶(MPO)、一氧化氮(NO)、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD);肝脏进行病理切片观察;酶联免疫法(ELISA)检测血清中白介素-1β(IL-1β)、白介素-10(IL-10)的表达水平。结果:与模型组相比,通体沉香醇提物能剂量依赖性显著降低血清ALT、AST活性,降低MPO活性、NO含量,提高T-AOC能力及SOD活性;缓解肝脏组织病理损伤;减低IL-1β和升高IL-10的表达水平,且通体沉香醇提物高剂量效果最好,优于等剂量的野生沉香和火烙沉香醇提物。结论:沉香醇提物对肝损伤有保护作用,其机制可能与抗氧化应激,抑制脂质过氧化及抗炎作用有关。Objective：This study is aimed to investigate the hepatoprotective effect of alcohol extract of agarwood on acute liver injury induced by carbon tetrachloride （CCl4） in mice.Methods：Mice were injected with CCl4 to establish acute liver injury model.Alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were measured of serum.The myeloperoxidase （MPO），nitric oxide （NO），total peroxide （T-AOC） and superoxide dismutase （SOD） of the liver tissue homogenate were measured，and observed degree of liver injury by pathological sections.The interleukin-1β （IL-1β） and Interleukin-10 （IL-10） expression levels were measured by enzyme-linked immunosorbent assay （ELISA）.Results：Compared with the model group，the alcohol extract of Agar-Wit agarwood groups significantly decreased the activities of serum ALT，AST，MPO and NO contents as well as the expression level of IL-1β，enhanced the capacity of T-AOC，the activity of SOD and IL-10 level，and improved pathological injury of liver tissue，especially the alcohol extract of Agar-Wit agarwood 2.84 g/kg group.Conclusion：Alcohol extracts of agarwood show a significantly protective effect on acute liver injury in mice，and its mechanism may be related to anti-oxidative stress，inhibition of lipid peroxidation and anti-inflammatory effect.

关 键 词：沉香醇提物 四氯化碳 急性肝损伤 抗氧化损伤 抗炎 

分 类 号：R575[医药卫生—消化系统]