左金丸对胃热证大鼠炎症因子、氧化应激因子及凋亡因子的影响  

Effect of Zuojin pill on inflammatory factors, oxidative stress factors and apoptosis factors in rats with stomach heat syndrome

作　　者：周祥羽 岳贺[1,2,3] 李春苑[1,2,3] 龚梦鹃[1,2,3] 王淑美[1,2,3] 梁生旺[1,2,3] 邹忠杰[1,2,3] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006 [3]广东高校中药质量工程技术研究中心,广东广州510006

出　　处：《中药与临床》2017年第2期49-52,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(81403075);广东省科技计划项目(2012B060300031)

摘　　要：目的:探讨左金丸干预大鼠胃热证的作用机制。方法:将大鼠随机分成正常组、模型组和左金丸组,通过灌胃辣椒煎液和无水乙醇建立大鼠胃热证模型,采用酶联免疫吸附法(ELISA)和化学比色法测定各组大鼠血清中的炎症因子如白细胞介素(IL)-2、IL-6、IL-10、IL-1β和髓过氧化物酶(MPO);氧化应激因子如还原型谷胱甘肽(GSH)、谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、一氧化氮(NO)、丙二醛(MDA)和凋亡因子如B细胞淋巴瘤因子-2(Bcl-2)的活性或含量。结果:左金丸能显著减少胃热证大鼠胃黏膜损伤面积,显著升高胃热证大鼠血清中GST、GSH-Px、CAT、SOD活性和GSH、IL-10、Bcl-2的含量,显著降低胃热证大鼠血清中MPO活性和IL-2、IL-6、IL-1β、MDA的含量。结论:左金丸可通过抗炎、抗氧化应激和抗凋亡作用干预胃热证。Objective： To explore the mechanisms of action of Zuojin Pill on stomach heat syndrome in rats. Method： The rats were randomly divided into normal group, model group and Zuojin pill group. Rat model of stomach heat syndrome was established by gavage of decoction of pepper and absolute ethanol. The contents or activities of inflammatory factors such as interleukin （IL）-2, IL-6, IL-10, IL-1β and myeloperoxidase （MPO） and oxidative stress factors such as glutathione （GSH）, glutathione-S-transferase （GST）, glutathione peroxidase （GSH-Px）, catalase （CAT）, superoxide dismutase （SOD）, nitric oxide （NO） and malondialdehyde （MDA） as well as apoptotic factors such as B-cell lymphoma-2 （Bcl-2） in rat serum were detected by chemical colorimetry and enzyme-linked immunosorbent assay （ELISA）. Result： Zuojin pill can significantly reduce gastric mucosal lesion areas of rat with stomach heat syndrome. Furthermore, Zuojin pill significantly increased the activities of GST, GSH-Px, CAT, SOD and the contents of GSH, IL-10, Bcl-2, as well as reduced the activity of MPO and the contents of IL-2, IL-6, IL-1β, MDA in serum of rats with stomach heat syndrome. Conclusion： Zuojin pill exerts its intervention effects on stomach heat syndrome by adjustment of inflammatory mediators and apoptotic markers and increasing the ability of anti-oxidative stress.

关 键 词：左金丸 胃热证 抗炎 抗氧化 抗凋亡 

分 类 号：R743.31[医药卫生—神经病学与精神病学]