铅暴露对大弹涂鱼(Boleophthalmus pectinirostris)组织形态的影响  

Effects of Pb^(2+) on tissue structure of liver,intestines and spleen in mudskipper Boleophthalmus pectinirostris

作　　者：景丹丹[1] 龚一富[1] 张燕[1] 俞凯[1] 黎明[1] 王日昕[1] JING Dan-dan GONG Yi-fu ZHANG Yan YU Kai LI Ming WANG Ri-xin(School of Marine Sciences, Ningbo University, Ningbo 315211, China)

机构地区：[1]宁波大学海洋学院,宁波315211

出　　处：《生物学杂志》2017年第4期29-32,共4页Journal of Biology

基　　金：国家自然科学基金(31272661);宁波市自然科学基金(2016A610083)

摘　　要：为了研究环境中铅离子(Pb^(2+))对大弹涂鱼肝、肠和脾组织结构的损伤,实验挑选体质量(21.80±2.70)g的健康大弹涂鱼96尾,分为4组,高剂量Pb^(2+)处理组(2 mg/L)、中剂量Pb^(2+)处理组(1 mg/L)、低剂量Pb^(2+)处理组(0.5 mg/L)和空白组,进行毒性试验,分别于第12 h、48 h和96 h取样,对各组织器官进行组织切片分析。结果表明:随着Pb^(2+)浓度的升高及处理时间的延长,肝组织出现组织水肿,部分细胞质空泡化、细胞核固缩;肠组织上皮细胞受损,杯状细胞明显增多;脾中淋巴细胞增多。研究表明,Pb^(2+)暴露对大弹涂鱼肝、肠及脾组织结构均会造成伤害。In order to study the effects of Pb2＋ on tissue structure of liver, intestines and spleen of fish, Mudskippers （21.80＆#177;2.70）g were randomly stocked into 16 tanks with 8 fish each in triplicate.The fish were exposed to 0, 0.5, 1 and 2 mg/L Pb2＋, and sampled on the 12th, 48th and 96th h.The results showed that Pb2＋ exposure results in tissue edema, cytoplasm vacuolization and in liver, epithelial cell damage, gobiet cells increased in intestinal, and lymphocytes increased in spleen.The results indicated that Pb2＋ exposure can cause damage to the structure of liver, intestines and spleen of mudskipper.

关 键 词：铅 大弹涂鱼 毒性机制 生物富集 组织结构 

分 类 号：Q495[生物学—生理学] X174[环境科学与工程—环境科学]