西红花苷在脑卒中后抑郁大鼠中抗炎作用的研究  

Anti-inflammatory effect of the crocin in post-stroke depression rats

作　　者：陈欣宇[1] 宋本华[1] 于洪[1] 浦岩柏[1] 聂文宝[1] 朱雨岚[2] 

机构地区：[1]齐齐哈尔市第一医院神经内科,黑龙江齐齐哈尔161000 [2]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2017年第3期223-227,共5页Journal of Harbin Medical University

摘　　要：目的探讨西红花苷对脑卒中后抑郁(post-stroke depression,PSD)大鼠的保护作用,并初步探讨其可能的作用机制。方法选用Wistar大鼠行卒中术后7天给予慢性不可预见性刺激结合孤养21天制作PSD模型。60只健康成年雄性Wistar大鼠,随机分为4组:卒中组、PSD组、PSD+西红花苷组和PSD+IL-1拮抗剂组。饲养30天后,分两方面进行检测:行为学方面行敞箱实验和糖水消耗实验检测;炎症指标利用Western blot法检测炎症因子IL-1的表达。结果行为学方面,与卒中组大鼠比较,PSD组大鼠糖水消耗量和敞箱实验中穿越格子数及站立次数明显减少(P<0.05);与PSD组大鼠比较,PSD+西红花苷组和PSD+IL-1拮抗剂组大鼠均能显著增加糖水消耗量和敞箱实验中穿越格子数及站立次数(P<0.05),但该两组间比较差异无统计学意义。炎症因子方面,与卒中组大鼠比较,PSD组大鼠脑组织中IL-1的表达有明显增加,差异有统计学意义(P<0.05);与PSD组大鼠比较,PSD+西红花苷组和PSD+IL-1拮抗剂组大鼠脑组织中IL-1的表达均显著降低,但该两组间差异无统计学意义。结论西红花苷可改善PSD大鼠的抑郁状态,其机制可能是通过抗炎作用实现的。Objective To investigate the effect of crocin on rats with post-stroke depression （PSD）, and to explore the mechanism of crocin in PSD. Methods The PSD model was built by Wistar rats after stroke operation in 7 days and treated with chronic, unpredictability stimu- lation for 3 weeks. Forty normal male Wistar rats were randomly divided into 4 groups： MCAO group, PSD group, PSD ＋ crocin group and PSD ＋ IL-1Ra group. After 30 days, the behavior score was observed by sucrose consumption and open field test. The expression of inflammatory factor IL-1 was detected by Western blot. Results Ethology： compared with MCAO group, the trend of sucrose consumption and the activity scores in open field test were decreased in PSD group （P 〈 0. 05 ） ; compared with PSD group, the trend of sucrose consumption and the activity scores in open field test were increased in PSD ＋ crocin group and PSD ＋ IL-1Ra group （ P 〈 0. 05 ）. Inflammatory factor： compared with MCAO group, the expression of IL-1 were increased in PSD group （P 〈 0. 05 ） ; compared with PSD group, the expression of IL-1 was de- creased in PSD ＋ crocin group and PSD ＋ IL-1Ra group （P 〈 0. 05 ）. Conclusion Crocin can improve the depression state of PSD rats by reducing inflammatory reaction.

关 键 词：西红花苷 脑卒中后抑郁 炎症 IL-1 

分 类 号：R749.12[医药卫生—神经病学与精神病学]