PGK1基因沉默对SMMC7721肝癌细胞增殖的影响  

Effect of PGK1 silencing on the proliferation of SMMC 7721 hepatoma cells

作　　者：张园园[1] 方肇勤[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《中西医结合肝病杂志》2017年第4期231-233,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金(No.81273641);中国博士后科学基金(No.2013M531201);上海市科委博士后科研基金项目(No.13R21415900);上海高校青年教师培养资助计划(No.ZZszy13004)

摘　　要：目的:观察PGK1基因沉默对SMMC7721肝癌细胞增殖的影响。方法:常规体外培养SMMC7721肝癌细胞,Lipofectamine 3000方法将sh RNA-PGK1质粒及阴性载体转染至SMMC7721细胞;荧光实时定量PCR及Western Blot检测PGK1的表达,MTT检测细胞相对存活率。结果:与正常SMMC 7721细胞相比,在PGK1沉默的肝癌细胞中,PGK1基因的表达降低,细胞的相对存活率减少。结论:PGK1基因沉默能够抑制SMMC7721肝癌细胞的增殖。： Objective：To investigate the effect of PGK1 gene silencing on the proliferation of SMMC7721 hepatoma cells. Methods： SMMC7721 hepatoma cells were cultured in vitro. Lipofectamine 3000 was used to transfect shRNA-PGK1 plasmid and SMMC7721 cells into SMMC7721 cells. The expression of PGK1 was detected by real-time fluorescence quantitative PCR and Western blot. Cell viability was measured by MTr. Results：Compared with normal 7721 cells, the expression of PGK1 in PGKl-silenced HCC cells was significantly decreased and the relative survival rate of PGK1 cells was decreased. Conclusion： PGK1 gene silencing can inhibit the proliferation of SMMC7721 hepatoma cells.

关 键 词：肝癌细胞 PGK1 RNA干扰 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]