真菌诱导子对龙眼胚性悬浮细胞类黄酮积累的影响  

Effects of Fungal Elicitors on the Accumulation of Flavonoids of Embryogenic Suspension Cells in Dimocarpus longan Lour.

作　　者：贺梦真 徐小萍[1] 张梓浩[1] 林玉玲[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《园艺与种苗》2017年第5期29-33,共5页Horticulture & Seed

基　　金：福建省大学生创新计划项目(201610389072);福建省重大科技专项(2015NZ-0002-1)资助项目

摘　　要：将拟茎点霉属、尖孢镰刀菌、胶孢镰刀菌3种真菌诱导子添加到龙眼悬浮培养体系中,研究不同种类真菌诱导子对龙眼胚性悬浮细胞类黄酮含量的影响。结果表明,龙眼胚性悬浮细胞在100 mg/L的拟茎点霉属真菌诱导子处理下诱导6 d后类黄酮的积累量最高,达到67.49%,说明拟茎点霉诱导子最有利于龙眼悬浮细胞类黄酮含量积累。In this experiment, the embryogenic suspension ceils were used as materials for studying the effects of elicitors from Phomopis Chi., Fusarium oxysporum, and Collectotrichum gloeospoHoides on the accumulation of flavonoids of embryonic suspension cells in Dimocarpus longan Lout., and the results showed that the highest content of flavonoids of 67.49% occurred on the treatment with 100 mg/L Phomopis Chi. elicitor on the 6th day after suspension culture, which suggested that the elicitor of Phomopis Chi. should be most beneficial to the accumulation of flavonoids in longan suspension ceils.

关 键 词：龙眼 胚性悬浮细胞 真菌诱导子 类黄酮 

分 类 号：S667.2[农业科学—果树学]