组蛋白去乙酰化酶抑制剂MS-275对卵巢癌SKOV3细胞增殖凋亡的影响  

Effect of histone deacetylase inhibitor MS-275 on proliferation and apoptosis of human ovarian cancer cell line SKOV3

作　　者：黄玉琴[1] 

机构地区：[1]湖北医药学院附属襄阳市第一人民医院,湖北襄阳441000

出　　处：《中国实验诊断学》2017年第8期1448-1451,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨MS-275对卵巢癌SKOV3细胞增殖凋亡的影响及其作用机制。方法 MTT法检测MS-275和顺铂单药及联合用药对SKOV3细胞增殖的影响,并计算半抑制浓度IC50;流式细胞术检测MS-275和顺铂单药及联合用药对SKOV3细胞凋亡的影响;Western-Blot法检测各组BCL2、survivin及p21蛋白的表达。结果 MS-275和顺铂两单药均可抑制SKOV3的增殖,各浓度组具有统计学差异(P<0.05),联合用药时可显著降低顺铂半抑制浓度(P<0.05);同时两单药组可促进SKOV3细胞凋亡(P<0.05),联合用药时凋亡作用更显著(P<0.05);MS-275和顺铂可显著抑制SKOV3细胞BCL-2和survivin蛋白的表达,增强p21的表达(P<0.05)。结论 MS-275抗SKOV3细胞增殖,促进其凋亡及增强顺铂敏感性的机制可能与抑制HDAC活性,抑制BCL-2和survivin蛋白表达,促进p21蛋白表达有关。Objective To investigate the effect of MS-275 on the proliferation and apoptosis and its mechanism of SKOV3 cells.Methods The proliferation inhibitory rates of SKOV3 cells treated with MS-275 or DDP alone and the combination of MS-275 and DDP were detected by MTT assay,and calculate the half inhibitory concentration（IC50）.The apoptosis rate of SKOV3 cells treated with MS-275 or DDP alone and the combination of MS-275 and DDP were determined by flow cytometry.The expression levels of BCL-2、survivin and p21 proteins in SKOV3 cells were examined by Western-Blotting.Results MS-275 and cisplatin alone could inhibit the proliferation of SKOV3,each concentration group has significant difference（P〈0.05）,United can significantly reduce the half inhibitory concentration of cisplatin（P〈0.05）.MS-275 or DDP alone can promote apoptosis of SKOV3 cell（P〈0.05）,MS-275 and cisplatin significantly inhibited the expression of BCL-2and surviving,and enhanced the expression of p21 in SKOV3 cells（P〈0.05）.Conclusion The mechanism of MS-275anti-SKOV3 cell proliferation,promoting apoptosis and enhancing cisplatin sensitivity may be related to inhibition of HDAC activity,inhibition of BCL-2 and survivin protein expression,and promotion of p21 protein expression.

关 键 词：MS-275 顺铂 卵巢癌 SKOV3 

分 类 号：R737.31[医药卫生—肿瘤]