麦秆碱预处理和同步糖化发酵工艺优化研究  

Optimization of alkali pretreatment and simultaneous saccharification and fermentation process of wheat straw

作　　者：崔茂金[1] 李长恭[1] 祝勇[1] 邓月娥[1] 

机构地区：[1]河南科技学院化学化工学院,河南新乡453003

出　　处：《中国油脂》2017年第8期98-102,共5页China Oils and Fats

摘　　要：通过响应面法和正交实验分别优化了麦秆的碱预处理工艺条件和同步糖化发酵工艺条件。首先以麦秆为底物通过Box-behnken设计研究了预处理温度、NaOH质量分数、预处理时间和底物质量浓度对总还原糖含量的影响;然后通过正交实验对碱预处理麦秆的同步糖化发酵工艺进行优化。结果表明:最佳碱预处理工艺条件为预处理温度137.64℃、NaOH质量分数6.72%、预处理时间41.93 min和底物质量浓度9.23 g/L,此时总还原糖含量最高,为496.00 mg/g,为未预处理底物的5.12倍,说明碱预处理可以较好地提高麦秆的糖化率;最佳同步糖化发酵工艺条件为发酵温度39℃、酵母接种量0.1%、酶质量浓度0.2 g/L和发酵时间2 d,此时乙醇含量最高,为22.84g/L。Response surface methodology and ortnogonat expcrlmcnt r ment conditions and simultaneous saccharification and fermentation conditions of wheat straw. Firstly, the effects of pretreatment temperature, NaOH mass fraction, pretreatment time and substrate mass concen- tration on the content of total reducing sugar were studied by Box - behnken design using wheat straw as substrate. Then simultaneous saceharification and fermentation conditions of wheat straw pretreated by al- kali were optimized by orthogonal experiment. The results showed that the optimal alkali pretreatment conditions of wheat straw were obtained as follows： pretreatment temperature 137.64 ℃, NaOH mass fraction 6.72%, pretreatment time 41.93 min and substrate mass concentration 9.23 g/L. The maximum content of total reducing sugar was 496.00 mg/g under the optimal alkali pretreatment conditions, which was 5.12 times of that from raw materials, indicating that alkali pretreatment was necessary to improve ethanol content was 22.84 g/L under the optimal simultaneous saccharification and fermentation conditions.

关 键 词：麦秆 碱预处理 同步糖化发酵 乙醇 优化 

分 类 号：S216.2[农业科学—农业机械化工程]