菊芋蛋白的提取及其降血糖作用研究  

Extraction of Helianthus Tuberosus Protein and Its Hypoglycemic Activity

作　　者：杨稳[1] 刘飞[1] 刘文静[1] 周长征[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医杂志》2017年第8期714-716,724,共4页Shandong Journal of Traditional Chinese Medicine

摘　　要：目的 :比较菊芋蛋白两种提取方法的蛋白质提取率,并做菊芋蛋白降血糖研究。方法 :菊芋采用丙酮沉淀法和硫酸铵盐析法提取蛋白质,以蛋白质含量为指标,优选蛋白质提取率高的提取方法,并通过小鼠体内降血糖实验,评价菊芋蛋白的降血糖效果。结果:丙酮沉淀法蛋白提取率为11.30%,硫酸铵盐析法提取率为10.80%;降糖作用研究表明,菊芋蛋白高剂量组降血糖作用明显。结论:丙酮沉淀法提取率高,菊芋蛋白具有降血糖的效果。Objective：To compare the extraction rate of two extraction methods for Helianthus tuberosus pro- tein,and to evaluate its antidiabetic effect. Methods Acetone precipitation and ammonium sulfate salting-out methods were applied for protein extraction,and protein content was applied as the index to optimize the extraction method,and the hypoglycemic activity was evaluated in the mouse. Results：The protein extraction rate by acetone precipitation was 11.30%,while that by ammonium sulfate salting-out method was 10.80%. The high dose of Helianthus tuberosus protein showed obvious hypoglycemic activity. Conclusion：Acetone sedimentation method is of high extraction rate,and Helianthus tuberosus protein is of hypoglycemic activity.

关 键 词：菊芋蛋白 丙酮沉淀法 硫酸铵盐析法 蛋白质电泳 紫外测含量 降血糖 

分 类 号：R284.2[医药卫生—中药学]