VdPKS1 is required for melanin formation and virulence in a cotton wilt pathogen Verticillium dahliae  

作　　者：Ting Zhang Bosen Zhang Chenlei Hua Pei Meng Sheng Wang Zhirong Chen Yejuan Du Feng Gao Jiafeng Huang 

机构地区：[1]College of Agriculture,Shihezi University and Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization,Shihezi 832003,China [2]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Science China(Life Sciences)》2017年第8期868-879,共12页中国科学（生命科学英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11040500);the National Natural Science Foundation of China(31160351,311060350);Basic Research Projects of Xingjiang Production and Construction Corps(2016AG001)

摘　　要：Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts. This pathogen survives for many years in soil in the form ofmelanized microsclerotia. To investigate the melanin synthesis in V.. dahliae, we identified a polyketide synthase gene in V. dahliae, namely VdPKS1. PKS1 is known to involve in the dihydroxynaphthalene melanin synthesis pathway in many fungi. We found that VdPKS1 was required for melanin formation but not for microsclerotial production in E dahliae. The VdPKS1 gene-disruption mutant （vdpksl） formed melanin-deficient albino microsclerotia, which did not affect the fungal colonization in host tissues but significantly reduced the disease severity. Gene transcription analysis in the wild-type and the vdpks1 strains suggested that VdPKS1 gene-disruption influenced the expression of a series of genes involved in ethylene biosynthesis, microsclerotial formation and pathogenesis. Our results suggest that the VdPKS1-mediated melanin synthesis is important for virulence and developmental traits of E dahliae.Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts.This pathogen survives for many years in soil in the form of melanized microsclerotia.To investigate the melanin synthesis in V.dahliae,we identified a polyketide synthase gene in V.dahliae,namely VdPKS1.PKS1 is known to involve in the dihydroxynaphthalene melanin synthesis pathway in many fungi.We found that VdPKS1 was required for melanin formation but not for microsclerotial production in V dahliae.The VdPKS1 gene-disruption mutant (vdpksl) formed melanin-deficient albino microsclerotia,which did not affect the fungal colonization in host tissues but significantly reduced the disease severity.Gene transcription analysis in the wild-type and the vdpksl strains suggested that VdPKS1 gene-disruption influenced the expression of a series of genes involved in ethylene biosynthesis,microsclerotial formation and pathogenesis.Our results suggest that the VdPKS1-mediated melanin synthesis is important for virulence and developmental traits of V.dahliae.

关 键 词：Verticillium dahliae VdPKS1 MELANIN VIRULENCE CONIDIATION MICROSCLEROTIA 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]