Differential immune responses of Monochamus alternatus against symbiotic and entomopathogenic fungi  

作　　者：Wei Zhang Jie Meng Jing Ning Peijun Qin Jiao Zhou Zhen Zou Yanhong Wang Hong Jiang Faheem Ahmad Lilin Zha Jianghua Sun 

机构地区：[1]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Biosciences,COMSATS Institute of Information Technology,Islamabad 45550,Pakistan [4]College of Life Science,Hebei University,Baoding 071002,China

出　　处：《Science China(Life Sciences)》2017年第8期902-910,共9页中国科学（生命科学英文版）

基　　金：supported by the Forestry Industry Research Special Funds for Public Welfare Project(201204501);National Key Plan for Scientific Research and Development of China(2016YFC1200604,2016YFD0500300);the High Technology Research and Development Program(HTRDP)of China(2014AA020529);National Natural Science Foundation of China(31572272,31370650,31402013,31221091,31672291,L1524009);the CAS Key Research Projects of the Frontier Science(QYZDBSSW-SMC014),and CAS(2015-SM-C-02)

摘　　要：Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.Monochamus alternates,the main vector beetles of invasive pinewood nematode,has established a symbiotic relationship with a native ectotrophic fungal symbiont,Sporothrix sp.1,in China.The immune response ofM.alternates to S.sp.1 in the coexistence of beetles and fungi is,however,unknown.Here,we report that immune responses ofM.alternates pupae to infection caused by ectotrophic symbiotic fungus S.sp.1 and entomopathogenic fungus Beauveria bassiana differ significantly.The S.sp.1 did not kill the beetles while B.bassiana killed all upon injection.The transcriptome results showed that the numbers of differentially expressed genes in M.alternates infected with S.sp.1 were 2-fold less than those infected with B.bassiana at 48 hours post infection.It was noticed that Toll and IMD pathways played a leading role in the beetle's immune system when infected by symbiotic fungus,but upon infection by entomopathogenic fungus,only the Toll pathway gets triggered actively.Furthermore,the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h.This study provided a comprehensive sequence resource ofM.alternates transcriptome for further study of the immune interactions between host and associated fungi.

关 键 词：Monochamus alternatus symbiotic fungus Beauveria bassiana RNA-SEQ immune signaling pathway 

分 类 号：S763[农业科学—森林保护学]