柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究  

Naringin inhibits the growth of non-small cell lung carcinoma cells through down-regulating p70S6K

作　　者：高中原 孙更[1] 李敏[1] 谌茜 李萍[1] 王雪融[1] 

机构地区：[1]南京医科大学基础医学院药理学系

出　　处：《中国临床药理学与治疗学》2017年第7期762-766,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81473241;81102458;81172004)

摘　　要：目的:研究柚皮苷对非小细胞肺癌细胞生长的作用及机制。方法:应用SRB法检测柚皮苷对非小细胞肺癌细胞株生长的抑制作用。用Western blot法检测柚皮苷对p70S6K的调控作用。利用质粒过表达p70S6K,用SRB法检测过表达p70S6K后对柚皮苷抑制细胞生长作用的影响。利用小干扰RNA阻抑p70S6K的表达,用SRB法检测阻抑p70S6K的表达对柚皮苷抑制细胞生长作用的影响。结果:柚皮苷呈浓度依赖性抑制非小细胞肺癌细胞的生长,并且可以下调p70S6K的表达。过表达p70S6K可以削弱柚皮苷抑制细胞生长的作用,敲降p70S6K可以增强柚皮苷抑制细胞生长的作用。结论:柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞的生长。AIM： To study the effect of naringin on the growth of non-small cell lung carcinoma cells （ NSCLC ） and its mechanism. METHODS ： SRB method was employed to investigate the inhibitory effect of naringin on the growth of non-small cell lung carcinoma cell lines. Western blot was used to detect the pTOS6K expression. The plasmid encoding full length p70S6K was transfected to over-express p70S6K. The small-interfering RNA was used to knockdown p70S6K expression. RESULTS：Naringin inhibited the growth of NSCLC cells, and down-regulated the expression of p70S6K. The over- expression of p70S6K partially abrogated the inhibitory effect of naringin, while knockdown of p70S6K enhanced the effect of naringin. CONCLUSION： Naringin can down-regulate the p70S6K to inhibit the growth of NSCLC cells.

关 键 词：P70S6K 柚皮苷 非小细胞肺癌细胞 

分 类 号：R734.2[医药卫生—肿瘤]