山楂降脂软胶囊中4种黄酮含量的测定  

Simultaneous quantification of four flavonoids in hawthorn lipid-lowering soft capsules

作　　者：杜娟[1] 左非非 魏双艳[1] 徐晓阳[1] 张玮杰 阴兆静 徐霞[1] 

机构地区：[1]郑州大学药学院,郑州450001

出　　处：《郑州大学学报（医学版）》2017年第4期456-459,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省基础与前沿技术基金资助项目142300410085;郑州市国际科技合作与交流项目153PGJHZ204

摘　　要：目的:建立同时测定山楂降脂软胶囊中4种主要黄酮葛根素、木犀草素、槲皮素及儿茶素的超高效液相-串联质谱法。方法:在超声条件下用甲醇提取山楂降脂软胶囊供试品。采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm)色谱柱分离,以体积分数0.1%甲酸乙腈和体积分数0.1%甲酸水溶液为流动相梯度洗脱,在负离子模式下采用多反应监测。结果:葛根素、木犀草素、槲皮素、儿茶素分别在0.03~8.10、0.04~8.27、0.02~4.45及0.01~3.11 mg/L的范围内线性良好,R^2均>0.992,4种黄酮的平均加样回收率在93.6%~107.5%,相对标准偏差在1.62%~2.21%。结论:建立了适用于快速测定山楂降脂软胶囊中4种黄酮的液质联用定量方法,该方法灵敏、准确、稳定,可用于山楂降脂软胶囊的质量控制。Aim：To develop a quantitative method for simultaneous determination of puerarin,mignonette,quercetin and catechin in hawthorn lipid-lowering soft capsules using ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry.Methods： Hawthorn lipid-lowering soft capsules were extracted with methanol under the condition of ultrasound.The separation was carried out on a Waters ACQUITY UPLC BEH C18（2.1 mm×50 mm,1.7 μm） column.And the mobile phase was consisted of acetonitrile-0.1%（V/V） formic acid and H2O-0.1%（V/V） formic acid in a gradient elution mode.Then the sample solution was analyzed in electrospray ionization-ion mode with multiple reaction monitoring.Results： The results suggested that puerarin,mignonette,quercetin and catechin exhibited a good linearity in the range of 0.03~8.10,0.04~8.27,0.02~4.45 and 0.01~3.11 mg/L,respectively.And the correlation coefficient was higher than 0.992.The average recovery rate of the 4 flavonoids was in the range of 93.6%~107.5%,while the range of relative standard deviation was 1.62%~2.21%.Conclusion： The established method is sensitive,accurate and stable,and meet the requirement for determination of the 4 major flavonoids in awthorn lipid-lowering soft capsules,thus could be used for quality control of hawthorn lipid-lowering soft capsules.

关 键 词：山楂降脂软胶囊 葛根素 木犀草素 槲皮素 儿茶素 液质联用 

分 类 号：R284.1[医药卫生—中药学]