血清β-2微球蛋白与炎症性肠病活动度关系的研究  

Serum beta 2-microglobulin—a biomarker of inflammatory bowel disease

作　　者：刘鹏[1] 黄美松 

机构地区：[1]湖北医药学院附属东风医院消化内科,湖北十堰442008 [2]十堰市中西医结合医院内分泌科,湖北十堰442011

出　　处：《临床消化病杂志》2017年第4期227-231,共5页Chinese Journal of Clinical Gastroenterology

摘　　要：[目的]研究炎症性肠病(IBD)患者血清中β-2微球蛋白(B2-M)含量与IBD活动度的关系,为B2-M用于诊断IBD活动度的生物标志物提供实验依据。[方法]酶联免疫法测定46例溃疡性结肠炎(UC)患者,34例克罗恩病(CD)患者和30例健康对照者血清中B2-M的含量。比较各组间B2-M和其他炎症标志物的含量并分析其含量与疾病活动度的关系。[结果]健康对照组、UC组、CD组血清中B2-M的平均含量分别为(1.85±0.63)、(2.59±0.54)、(2.32±0.44)mg/L;UC组患者血清中B2-M含量≥1.99mg/L时,其灵敏度、特异性、阳性预测值(PPV)和阴性预测值(NPV)分别为64%、78%、83%和62%。CD组患者血清中B2-M含量≥1.70mg/L时,其灵敏度、特异性、PPV和NPV分别为71%、52%、73%和53%。UC组和CD组患者血清中B2-M的含量均显著高于健康对照组(P<0.01和P<0.05);与缓解期UC患者、CD患者比较,活动期UC患者、CD患者血清中B2-M的含量明显增加[UC组:(2.87±0.53)∶(2.01±0.32)mg/L,P<0.01;CD组:(2.73±0.44)∶(1.85±0.35)mg/L,P<0.05]。UC组与CD组患者血清中B2-M的含量比较差异无统计学意义[(2.59±0.54)∶(2.32±0.44)mg/L,P>0.05]。[结论]血清中B2-M的含量可作为诊断IBD和评价IBD疾病活动度的生物标志物。[Objective]To investigate the correlation between beta 2 microglobulin（B2-M）levels and the activity of inflammatory bowel disease（IBD）,and provide theory basis for B2-M regard as a biomarker to diagnose IBD and evaluate the activity of IBD. [,Methods]To determine serum B2-M levels in 43 ulcerative colitis（UC）patients, 35 with Crohn＇s disease（CD）and 30 control subjects（Con）using an enzymatic method. IBD patients were divided into active and remission according to two disease types. Patients were also divid- ed into left-side and pancolitis for UC and ileitis and ileocolitis for CD according to extent of the disease. All groups mean serum B2-M levels and other inflammatory markers levels were compared and analyze correla- tion between serum B2-M levels and activity of IBD. [Results]The mean serum B2-M levels in the Con,UC and CD patients were （1.85±0.63）,（2.59±0.54） and （2.32±0.44） mg/L respectively. B2-M levels 1.99 mg/L had 62% sensitivity,76% specificity, 79% positive predictive value（PPV）and 58% negative predictive value（NPV） for UC patients. B2-M levels ≥1.70 mg/L had 80% sensitivity, 53% specificity, 66% PPV,and 69% NPV for CD patients. Mean B2-M levels were significantly higher in UC and CD pa- tients than in healthy Con（UC： [2.59±0.54] vs [-1.85±0.63]mg/L,P〈0.01;CD：[2. 32±0.44] vs [-1.85 ±0.63]mg/L,P〈0. 05）. Also,compared with remission IBD,mean B2-M levels of active IBD were significantly higher（UC：[2.87±0.53] vs [2.01±0. 322mg/L,P〈0.01;CD：[2.73±0. 441 vs [1.85±0.35]mg/ L, P〈0.05）. The difference between UC and CD groups in terms of serum B2-M levels was not statistical- ly significant（F2.59 ±0.54] vs [2.32 ± 0.44]mg/L, P〉0.05）. [Conelusion]Serum B2-M levels may be used as a biomarker to diagnose IBD and evaluate the activity of IBD.

关 键 词：炎症性肠病 Β-2微球蛋白 克罗恩病 溃疡性结肠炎 生物标志物 

分 类 号：R574[医药卫生—消化系统]