基于单克隆抗体的食品中乳酸链球菌素酶联免疫方法  

Enzyme linked immunosorbent assay for quantitative detection of Nisin based onmonoclonal antibody food

作　　者：张世伟[1] 冯荣虎[1] 王士峰[1] 赖心田[1] 杨国武[1] 

机构地区：[1]深圳市计量质量检测研究院,深圳518102

出　　处：《中国食品添加剂》2017年第8期166-169,共4页China Food Additives

摘　　要：建立了乳酸链球菌素的酶联免疫检测方法。以乳酸链球菌素A偶联牛血清白蛋白作为抗原制备了单克隆抗体,该抗体能等同识别乳酸链球菌素A和Z,对其他多肽类抗生素无交叉反应。所建立的乳酸链球菌素检测方法 IC_(50)为0.49μg/mL,线性范围为0.05μg/mL^5.4μg/mL(y=-0.12ln(x)+0.385,R^2=0.98)。该方法适用于热加工样品的检测,前处理简单,整个检测过程仅需60min,为乳酸链球菌素速测提供了有力的技术手段。Anenzyme linked immunosorbent assay（ELISA）for quantitative detection of nisin was established.Nisin A coupled with bovine serum albumin（BSA）was used as antigen to prepare monoclonal antibody. The antibody could combine with Nisin A and Nisin Z equally and did not combine with other polypeptide antibiotics. The assay exhibited dynamic linear range for detection of Nisin from 0.05 μg/mL to 5.4 μg/mL（y =-0.12ln（x）＋ 0.385,R2= 0.98）,IC（50） was 0.49 ng/mL. The assay method could be applied in heat-processed products. The detection time for each sample was 60 min.

关 键 词：乳酸链球菌素 单克隆抗体 酶联免疫分析 定量 

分 类 号：TS202.3[轻工技术与工程—食品科学]