基于UPLC-Q-TOF/MS技术的北青龙衣褐变过程中成分动态变化分析  

Dynamic variation of components in exocarp of Juglans mandshurica with browning based on UPLC-Q-TOF/MS

作　　者：孙国东 霍金海 谢荣娟 王伟明 

机构地区：[1]黑龙江省中医药科学院中药研究所,黑龙江哈尔滨150036

出　　处：《中国中药杂志》2017年第16期3112-3120,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81503348);哈尔滨市青年科技创新人才基金项目(2012RFOYSO42)

摘　　要：该文分析不同褐变时期北青龙衣有效成分动态变化规律。采用UPLC-Q-TOF-MS/MS技术对13个褐变时期26批样品进行测定,差异性化合物通过Peakview 2.0/masterview1.0软件,依据精确质量数和同位素峰度比确定分子式,再通过与对照品及质谱数据库的二级谱图比对、裂解规律分析,并结合已有文献报道,确定结构式。结果不同褐变时期化学成分含量发生了明显的变化,鉴定或推断了北青龙衣中25个化合物结构,随着褐变时间的变化,13个萘醌类化合物中,具有胡桃醌相似母核的6个化合物及3个萘醌苷类化合物的含量显著下降,产生胡桃酮等4个萘醌衍生物;4个黄酮及2个酚酸类化合物含量显著下降;6个二芳基庚烷类化合物显著提高。该方法能够快速、准确鉴定北青龙衣的化学成分,并分析动态变化规律,为揭示褐变对中药材及果蔬成分的影响提供一种新的研究策略。To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods. Twentysix batches of exocarp of J. mandshurica samples from thirteen browning periods were assessed by UPLC-Q-TOF-MS/MS. The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 2. 0/masterview1. 0 software. Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references. The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred. Of the 13 naphthoquinone compounds,the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly,and 4 naphthoquinone derivatives such as regiolone were produced; the contents of four flavones and two phenolic acids compounds were decreased significantly; and the contents of 6 diarylheptanoids compounds were increased significantly. UPLC-Q-TOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately,and analyze the rules of dynamic changes,to reveal the browning of Chinese medicinal materials and its effects on compositions of fruits and vegetables.

关 键 词：液相色谱-四极杆飞行时间质谱 北青龙衣 褐变 动态变化 

分 类 号：R284.1[医药卫生—中药学]