围术期静脉注射利多卡因对宫颈癌病人淋巴细胞免疫功能的影响  

EFFECT OF PERIOPERATIVE INTRAVENOUS INJECTION OF LIDOCAINE ON LYMPHOCYTE IMMUNE FUNCTION IN PATIENTS WITH CERVICAL CANCER

作　　者：王焕亮[1,2] 刘亚洋[3] 王欢[4] 类维富[2] 

机构地区：[1]山东大学深圳研究院,广东深圳518057 [2]山东大学齐鲁医院麻醉科 [3]山东中医药大学附属医院麻醉科 [4]济南市妇幼保健院病案室

出　　处：《齐鲁医学杂志》2017年第3期276-278,共3页Medical Journal of Qilu

基　　金：深圳市未来产业专项资金项目(JCYJ20150402-05524051);山东省自然科学基金项目(ZR2015HM038)

摘　　要：目的探讨围术期静脉输注利多卡因对宫颈癌病人外周静脉血淋巴细胞免疫功能的影响。方法择期行宫颈癌根治术病人30例,随机分为对照组(C组)和利多卡因组(L组)。L组病人于麻醉诱导前15 min静脉注射利多卡因1.5mg/kg,随后以微量泵静脉输注利多卡因1.5mg·kg-1·h-1至手术结束;对照组给予等量的生理盐水。分别于术前24h、术后即刻、术后48h采集外周静脉血10mL,分离血浆和淋巴细胞。应用流式细胞仪测定外周血淋巴细胞凋亡率,采用CCK-8法测定外周血淋巴细胞增殖反应,采用ELISA法测定血浆干扰素γ(IFN-γ)和白细胞介素4(IL-4)水平。结果与C组比较,L组病人术后即刻、术后48h淋巴细胞增殖能力升高(F=4.32、4.65,P<0.05)。术后48h,L组病人血浆IFN-γ水平和IFN-γ/IL-4均高于C组(F=7.581、5.273,P<0.05)。结论利多卡因围术期静脉输注可保护宫颈癌手术病人淋巴细胞免疫功能,有助于病人术后康复。Objective To investigate the effect of perioperative intravenous infusion of lidocaine on immune function of lymphocytes in peripheral venous blood in patients undergoing surgery for cervical cancer. Methods A total of 30 patients who underwent radical surgery for cervical cancer were randomized into control group（group C）and lidocaine group（group L）.The patients in group L were given intravenous injection of lidocaine 1.5mg/kg at 15 minutes before induction of anesthesia,followed by intravenous infusion of lidocaine 1.5mg·kg-1·h-1 using a micro-pump till the end of surgery,and those in group C were given an equal volume of normal saline.A total of 10 mL peripheral venous blood was collected at 48 hours before surgery,immediately after surgery,and at 48 hours after surgery,and plasma and lymphocytes were isolated.Flow cytometry was used to measure the apoptosis rate of peripheral lymphocytes,CCK-8was used to measure the proliferation of peripheral lymphocytes,and ELISA was used to measure the plasma levels of interferon gamma（IFN-γ）and interleukin-4（IL-4）. Results Compared with group C,group L had a significant increase in the proliferative capacity of lymphocytes immediately and at 48 hours after surgery（F=4.32,4.65;P〈0.05）.At 48 hours after surgery,group L had significantly higher plasma level of IFN-γand IFN-γ/IL-4ratio than group C（F=7.581,5.273;P〈0.05）. Conclusion Perioperative intravenous infusion of lidocaine exerts a protective effect on the immune function of lymphocytes in patients with cervical cancer undergoing radical surgery and may bring benefits to postoperative rehabilitation.

关 键 词：宫颈肿瘤 利多卡因 淋巴细胞 干扰素Γ 白细胞介素4 

分 类 号：R737.33[医药卫生—肿瘤] R971.2[医药卫生—临床医学]