miR-551b在人胃癌组织中的表达及对胃癌细胞凋亡的影响  

Expression of miR-551b in Human Gastric Carcinoma and Its Effect on Apoptosis of Human Gastric Carcinoma Cell Lines

作　　者：王娅南[1] 魏亚宁[2] 徐芳[1] 范翔宇[1] 张培[1] 李赟[1] 王盼[1] 张金库 郑红婧 

机构地区：[1]河北大学附属医院病理科,保定071000 [2]河北大学附属医院肿瘤内科,保定071000 [3]河北省保定第一中心医院病理科,保定071028 [4]河北省保定市第七人民医院病理科,保定072150

出　　处：《华中科技大学学报（医学版）》2017年第4期386-391,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81672706)

摘　　要：目的探讨miR-551b在人胃癌组织中的表达及对胃癌细胞凋亡的影响。方法采用Real-time PCR检测胃癌组织、正常胃组织miR-551b表达水平,分别将miRNA无义序列、miR-551bmimics、miR-551binhibitors转染至人胃癌细胞株SGC-7901,Real-time PCR检测各组细胞miR-551b表达,MTT法检测各组肿瘤细胞增殖活性,Transwell法检测各组细胞侵袭能力,流式细胞术检测各组细胞凋亡情况,Hoechst33342荧光染色观察各组细胞自噬、凋亡的发生,Western blot检测各组细胞NF-κB、LC3Ⅱ、Beclin 1蛋白表达。结果 miR-551b在胃癌组织表达明显下调,胃癌组织miR-551b相对表达量(1.75±0.13)显著低于正常胃组织(2.47±0.38)(P<0.05)。与NC组和miR-551binhibitors组比较,miR-551bmimics组miR-551b表达水平显著上升,细胞增殖率和侵袭率显著降低,细胞凋亡率明显上升,各指标比较差异均具有统计学意义(均P<0.05)。Hoechst33342荧光染色显示miR-551bmimics组出现大量自噬泡,NC组可见部分自噬泡,而miR-551binhibitors组仅有少量自噬泡。Western blot检测结果显示,与NC组和miR-551binhibitors组比较,miR-551bmimics组NF-κB、LC3Ⅱ、Beclin 1蛋白表达水平显著升高(均P<0.05)。结论 miR-551b能够抑制胃癌细胞的增殖和侵袭等细胞生物功能,其作用机制可能是诱导了胃癌细胞发生自噬性凋亡。Objective To explore miR-551 bexpression in human gastric carcinoma and effect of miR-551 bon autophagic apoptosis of human gastric carcinoma cell lines.Methods The miR-551 bexpression in gastric cancer tissues and normal gastric tissues was detected by real-time PCR.miRNA nonsense sequences,miR-551 bmimics,miR-551 binhibitors were transfected into human gastric carcinoma SGC-7901 cell lines,and miR-551 bexpression in the cells of each group was detected by real-time PCR.Proliferation activity of tumor cells was detected by MTT assay;invasion ability of tumor cells was detected by Transwell method;cell apoptosis was detected by flow cytometry;autophagy and apoptosis in each group was observed by Hoechst33342 fluorescence staining;protein expression of NF-κB,LC3Ⅱ and Beclin 1was detected by Western blotting.Results The expression of miR-551 bin gastric carcinoma was significantly down-regulated,and the relative expression of miR-551b（1.75±0.13）in gastric carcinoma was significantly lower than that in normal gastric tissue（2.47±0.38）（P0.05）.Compared with NC group and miR-551 binhibitors group,miR-551 bexpression level in miR-551 bmimics group was significantly increased,proliferation rate and invasion rate were significantly decreased,apoptosis rate significantly increased（all P0.05）.Hoechst33342 fluorescence staining showed that large number of autophagy bubbles appeared in miR-551 bmimics group,some autophagy bubbles in NC group,and only a small number of autophagy bubbles in miR-551 binhibitors group.Western blotting showed that,as compared with NC group and miR-551 binhibitors group,expression levels of NF-κB,LC3Ⅱand Beclin 1in miR-551 bmimics group were significantly increased（P0.05）.Conclusion MiR-551 bcan inhibit the proliferation and invasion of gastric cancer cells,and the action mechanism may be related to inducing the autophagic apoptosis of gastric cancer cells.

关 键 词：微小RNA 胃癌 侵袭、凋亡 增殖 自噬性凋亡 

分 类 号：R735.2[医药卫生—肿瘤]