上皮-间质转化调控蛋白在前列腺癌侵袭、转移中的作用及其临床预后诊断价值  

Effect of Epithelial-mesenchymal Transition Regulation Protein in PCa Invasion and Metastasis and Its Diagnostic Value for Clinical Prognosis

作　　者：周毅[1] 姚远[1] 杨剑文[1] 卢启海[1] 陈翔[1] 陈悦康[1] 

机构地区：[1]广西科技大学附属柳州市人民医院泌尿外科,柳州545006

出　　处：《华中科技大学学报（医学版）》2017年第4期397-403,共7页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(No.Z2014435)

摘　　要：目的探讨上皮-间质转化(EMT)调控蛋白在前列腺癌(PCa)侵袭、转移中的作用及其对临床预后的诊断价值。方法收集48例PCa组织样本(PCa组)和50例良性前列腺增生(BPH)组织样本(BPH组),免疫组化SP法检测样本神经钙黏素(N-cadherin)、上皮钙黏素(E-cadherin)表达,分析N-cadherin、E-cadherin表达与PCa患者临床资料的关系。对PCa患者进行随访,记录患者总生存期(OS);以OS作为评价指标,采用单变量和多变量Cox比例风险模型评价患者预后的影响因素。以转化生长因子β1(TGF-β1)诱导DU-145细胞发生EMT,细胞增殖实验检测细胞增殖能力,Transwell实验检测细胞迁移能力,Western blot检测细胞E-cadherin、N-cadherin蛋白表达。结果 PCa组N-cadherin表达水平显著高于BPH组,E-cadherin表达水平显著低于BPH组(均P<0.05)。肿瘤直径≥2.5cm者N-cadherin高表达率显著高于肿瘤直径<2.5cm者,Ⅳ期PCa患者N-cadherin高表达率显著高于Ⅱ期和Ⅲ期PCa患者,淋巴结转移者N-cadherin高表达率显著高于无淋巴结转移者,远处转移者N-cadherin高表达率显著高于无远处转移者(均P<0.05)。Gleason分级≥7分者E-cadherin高表达率显著低于Gleason分级≤6分者,有淋巴结转移者E-cadherin高表达率显著低于无淋巴结转移者,有远处转移者E-cadherin高表达率显著低于无远处转移者(均P<0.05)。N-cadherin高表达患者OS显著低于N-cadherin低表达患者(P=0.024),E-cadherin高表达患者OS显著高于E-cadherin低表达患者(P=0.017)。单因素和多因素分析显示,T4期肿瘤、N-cadherin高表达、E-cadherin低表达是影响患者OS的独立危险因素(均P<0.05)。细胞增殖实验显示,第24~96h,DU-145组吸光度值显著高于NC组(均P<0.05);Transwell实验显示,DU-145组穿膜细胞数量显著多于NC组(P<0.05);Western blot实验显示,DU-145组N-cadherin蛋白表达水平显著高于NC组,E-cadherin蛋白表达水平显著低于NC组(均P<0.05)。结论 EMT与PCa的增殖、侵袭和�Objective To explore the effect of epithelial-mesenchymal transition（EMT）regulation protein in prostate cancer（PCa）invasion and metastasis and its diagnostic value of clinical prognosis.Methods Totally,48 cases of PCa tissue samples（PCa group）and 50 cases of benign prostatic hyperplasia（BPH）tissue samples（BPH group）were collected.Expression levels of N-cadherin and E-cadherin were detected by immunohistochemical SP method in tissue samples,the relationship between N-cadherin,E-cadherin and clinical data in PCa patients was analysed.PCa patients were followed up,the overall survival（OS）was recorded,with OS serving as evaluation index.The prognostic factors were evaluated by univariate and multivariate Cox proportional hazards model.EMT was induced by TGF-β1in DU-145 cells line.The cell proliferation ability was detected by cell proliferation assay.Cell invasion and migration ability was detected by Transwell.E-cadherin and N-cadherin protein expression was detected by Western blotting.Results In PCa group,the expression of N-cadherin was significantly higher than that in the BPH group,and the expression of E-cadherin was significantly lower than that in the BPH group（both P0.05）.The N-cadherin high expression rate was significantly higher in tumor with diameter≥2.5cm than that in the tumor with diameter2.5cm;Ncadherin high expression rate in stageⅣPCa patients was significantly higher than that in stageⅡandⅢ PCa patients;the high expression rate of N-cadherin was significantly higher in patients with lymph node metastasis than in those without lymph node metastasis;the high expression rate of N-cadherin was significantly higher in patients with distant metastasis than in those with-out distant metastasis（all P0.05）.The E-cadherin high expression rate was significantly lower in patients with Gleason score≥7than in those with Gleason score≤6;the high expression rate of E-cadherin was significantly lower in patients with lymph node metastasis than in those without lymph no

关 键 词：上皮-间质转化 神经钙黏素 上皮钙黏素 前列腺癌 增殖 侵袭 预后 

分 类 号：R737.25[医药卫生—肿瘤]