海马区血小板活化因子合酶及其受体的表达对选择性神经损伤大鼠痛觉敏化的影响  

Roles of Platelet-activation Factor Synthases and Its Receptor in Neuropathic Pain Induced by Spared Nerve Injury in Hippocampus of Rats

作　　者：杨京利[1] 张力[2] 向勇[2] 马国平 

机构地区：[1]复旦大学附属浦东医院麻醉科,上海201399 [2]湖北医药学院附属太和医院疼痛科,十堰442000 [3]上海市浦东新区周浦医院麻醉科,上海201318

出　　处：《华中科技大学学报（医学版）》2017年第4期459-461,497,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省教育厅科研基金资助项目(No.D20132405);上海市浦东新区卫生人才培养计划资助项目(No.PWRd2014-19)

摘　　要：目的探讨海马区血小板活化因子(PAF)合酶(LPCAT1、LPCAT2)及其受体(PAFR)在大鼠坐骨神经分支选择性神经损伤(SNI)所致神经病理性疼痛中的作用。方法选择海马区置管成功的雄性SD大鼠40只,随机分为4组(n=10):假手术组(Sham组),模型组(SNI组),模型+银杏内酯B组(SNI+BN52021组)和溶剂对照组(SNI+HBC组)。SNI+BN52021组在SNI手术后2d通过海马区微量注射给予银杏内酯B 10μg(溶解于1μL的HBC溶液),SNI+HBC组则给予1μL的HBC溶液。分别于术前1d和术后1、3、5、7、10、14d记录各组动物的机械缩爪阈值(PWMT)和辐射热缩爪潜伏期(PWTL);第15天取大鼠新鲜海马组织,RT-PCR检测LPCAT1、LPCAT2以及PAFR的表达。结果SNI大鼠PWMT值术后第1天开始明显降低(P<0.05),海马组织中LPCAT2及PAFR的表达增强(均P<0.05),LPCAT1的表达变化不明显(P>0.05);海马区微量注射BN52021组大鼠的PWMT值升高,海马组织中LPCAT2及PAFR的表达下降(均P<0.05)。结论海马区微量注射银杏内酯B可减轻SNI大鼠的机械性痛敏,抑制海马区LPCAT2和PAFR的表达,海马区PAF/PAFR信号通路可能参与了SNI大鼠神经病理性疼痛机制。Objective To investigate the roles of platelet-activating factor（PAF）synthases（LPCAT1and LPCAT2）and its receptor（PAFR）in neuropathic pain induced by spared nerve injury（SNI）in hippocampus.Methods Totally,40 SD rats with the cannula stereotaxically implanted into the dorsal hippocamus were randomly divided into 4groups：Sham group,SNI group,Ginkgolide B group（SNI＋BN52021group）and vehicle control group（SNI＋HBC group）.The rats in SNI＋BN52021group were administered by intrahippocampal injection with Ginkgolide B 10μg/μL 2days after SNI surgery,and the rats in SNI＋HBC group were administered by intrahippocampal injection with 45% HBC 1μL.The paw withdrawal mechanical threshold（PWMT）and paw withdrawal thermal latency（PWTL）were recorded 1day before surgery and 1,3,5,7,10 and 14days after surgery.The expression of PAF synthases（LPCAT1and 2）and PAF receptor（PAFR）in hippocampus were detected by RTPCR.Results PWMT decreased significantly 1day after surgery in SNI group（P0.05）.The expression levels of LPCAT2 and PAFR were increased significantly in hippocampus in SNI group（P0.05）.PWMT increased significantly in SNI rats administered by intrahippocampal injection with Ginkgolide B（P0.05）,and the expression of LPCAT2 and PAFR decreased significantly in hippocampus in SNI＋BN52021group（P0.05）.Conclusion Intrahippocampal injection with Ginkgolide B could decrease the expression of LPCAT2 and PAFR,and alleviate mechanical hyperalgesia.It suggests that PAF/PAFR in hippocampus participates in the mechanism of neuropathic pain after SNI surgery.

关 键 词：银杏内酯B 神经病理性疼痛 海马 血小板活化因子 

分 类 号：R745.4[医药卫生—神经病学与精神病学]