叉头蛋白M1在食管鳞状上皮异型增生至癌变过程中的表达及其与临床病理的相关性  

FOXM1 expression in the process of dysplasia and carcinogenesis of esophageal epithelium and its correlation with clinicopathological characteristics

作　　者：刘春涛[1] 姜大磊[2] 张政[1] 王拥军[1] 李鹏[1] 张澍田[1] 

机构地区：[1]首都医科大学附属北京友谊医院消化内科国家消化系统疾病临床医学研究中心北京市消化疾病中心,北京100050 [2]青岛市市立医院消化科,山东青岛266011

出　　处：《临床和实验医学杂志》2017年第17期1668-1672,共5页Journal of Clinical and Experimental Medicine

基　　金：北京市优秀人才培养资助项目(2015000021469G233);北京市医院管理局“青苗”计划专项(QML20150105);首都医科大学基础-临床科研合作基金资助项目(15JL30);北京友谊医院科研启动基金资助项目(yyqdkt2013-7)

摘　　要：目的检测食管癌前病变组织、食管鳞癌组织及癌旁正常食管鳞状上皮中叉头蛋白M1(FOXM1)的表达水平,探讨FOXM1蛋白表达水平与患者临床病理特征的相关性。方法收集60例食管癌前病变组织(包括低级别瘤变和高级别瘤变各30例)和115例食管鳞状细胞癌及配对的癌旁组织标本,所有病例均经病理证实,同时收集患者的临床资料。采用免疫组化的方法检测组织中FOXM1的表达情况。结果 FOXM1蛋白主要表达于肿瘤细胞及癌前病变细胞中,在细胞浆与细胞核中均有表达。FOXM1在食管低级别瘤变、高级别瘤变和食管鳞癌组织中的高表达率分别为26.7%(8/30)、57.7%(17/30)和60.0%(69/115),而在配对癌旁组织中呈低表达或无表达。FOXM1异常表达率增高与食管鳞状细胞癌患者的肿瘤大小、肿瘤浸润深度及肿瘤TNM分期存在显著相关性(分别为P<0.001,P<0.001和P=0.032),而与患者的性别、年龄、肿瘤细胞分化程度、淋巴结转移等无明显相关性(P>0.05)。结论 FOXM1在食管鳞状上皮癌前病变阶段即出现表达升高,且随着食管鳞状上皮癌变的发生发展表达逐渐升高。FOXM1的异常表达与食管鳞癌患者的肿瘤大小、浸润深度及肿瘤TNM分期存在显著相关性。Objective To detect the expression of FOXM1 in esophageal pre - malignant lesions, esophageal squamous cell carcinoma （ESCC） tissues and paired non- cancerous tissues, and to investigate the association between FOXM1 and clinicopathological characteristics of ESCC patients. Methods Sixty esophageal pre - malignant lesions （ including 30 cases of low grade and high grade intra - epithelial neoplasia re- spectively）, 115 pairs of ESCC tissues and paired non -cancerous esophageal tissues were collected. All cases were confirmed by pathology. Pa- tients＂ clinical characteristics were collected meanwhile. Immunohistochemistry （IHC） was applied to detect the expression of FOXM1 in the tis- sues. Results FOXM1 protein was mainly expressed in tumor cells and pre - malignant lesion cells and was expressed in both cytoplasm and nu- cleus. The expression of FOXM1 in non - cancerous esophageal tissues was weak or negative, whereas strong positive staining of FOXM1 was ob- served in low grade intra -epithelial neoplasia （8/30, 26.7% ）, high grade intra -epithelial neoplasia （17/30, 57.7% ）, and ESCCs （69/115, 60.0% ）. The expression of FOXMI was correlated with tumor size, tumor invasion depth, and TNM stage （ P 〈0. 001, P 〈0. 001, P =0. 032, respectively） in ESCC patients. No significant association between FOXM1 expression and other elinicopathologieal parameters （patients＇gender, age, cell grading, and lymph node metastasis） was found （ P 〉 0.05 ）. Conclusion The overexpression of FOXM1 emerges in the stage of e- sophageal pre -malignant lesion, and the expression increases gradually with the development of ESCC. The overexpression of FOXM1 is correla- ted with tumor size, tumor invasion depth, and TNM stage of ESCC patients.

关 键 词：鳞状上皮异型增生 食管鳞癌组织 蛋白表达水平 相关性 临床病理 癌变过程 鳞状细胞癌患者 癌前病变组织 

分 类 号：R735.1[医药卫生—肿瘤]