非神经元型胆碱能系统在重症肌无力患者外周血单个核细胞中的表达  

The expression of non - neuronal cholinergic system in peripheral blood mononuclear cells of patients with myasthenia gravis

作　　者：刘凡昭 崔新征[1] 朱利伟[2] 张清勇[1] 陆增辉[1] 张迎娜[3] 高峰[3] 陈嘉捷 

机构地区：[1]郑州大学第二附属医院胸外科,450014 [2]漯河医学高等专科学校第三附属医院外科,462000 [3]郑州大学医药科学研究院神经免疫学研究室,450052

出　　处：《中华实验外科杂志》2017年第9期1492-1495,共4页Chinese Journal of Experimental Surgery

基　　金：河南省科技厅科技攻关项目（162102310505、172102310278）

摘　　要：目的观察非神经元型胆碱能系统（NNCS）在重症肌无力（MG）患者外周血单个核细胞（PBMC）中的表达。 方法取MG患者及对照组受检者肘正中静脉血，分离出单个核细胞，提取总RNA，反转录-聚合酶链反应（RT-PCR）检测毒蕈碱型乙酰胆碱受体（mAChR：M1R～5R）、烟碱型乙酰胆碱受体（nAChR：α2～10、β2～4）、胆碱乙酰转移酶（ChAT）和胆碱酯酶（AChE）的表达。通过实时定量聚合酶链反应（Real-time PCR）定量分析mAChR（M1R、M3R、M5R）、nAChR（α5、α7）以及AChE在对照组和MG组中的差异。结果RT-PCR结果显示MG组和对照组PBMC中均表达M1R、M3R、M5R、α5、α7和AChE，但nAChR（α3、α9、β2）只在对照组中检测出，其余各亚型和ChAT在两组均未见表达。Real-time PCR结果显示MG患者PBMC中M1 mAChR表达量为对照组的0.80倍（P＝0.418），M3 mAChR为对照组的0.40倍（P＝0.004），M5 mAChR为对照组的1.23倍（P＝0.488），α5 nAChR为对照组的0.63倍（P＝0.177），α7 nAChR为对照组的0.81倍（P＝0.668），AChE为对照组的3.24倍（P＝0.002）。结论 MG患者PBMC中nAChR（α3、α9、β2）、M3 mAChR以及AChE表达存在差异。Objective To detect the expression of non-neuronal cholinergic system （NNCS） in peripheral blood mononuclear cells （PBMCs） in patients with myasthenia gravis （MG）. Methods The middle elbow vein blood was drawn from MG patients and controls, and the total RNA was extracted from isolated mononuclear cells. The expression of muscarinic acetylcholine receptor （mAChR） subtypes （M1R, M2R, M3R, M4R, M5R）, nicotinic acetylcholine receptor （nAChR） subtypes （α2, α3, α4, α5, α6, α7, α9, α10, β2, β3, β4）, choline acetyltransferase （ChAT） and cholinesterase （AChE） was detected by reverse transcription polymerase chain reaction （RT-PCR）. At last, mAChR （M1R, M3R, M5R）, nAChR （α5, α7） and AChE in control group and MG group were quantitatively analyzed by real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. Results RT-PCR results showed that PBMCs in both MG group and control group expressed M1R, M3R, M5R, α5, α7 and AChE, but nAChR （α3, α9, β2） was only detected in the control group, and the rest of the subunits and ChAT in the two groups were not expressed. Real-time PCR analysis showed that the expression of M1 mAChR in MG patients was 0.80 times higher （P=0.418）, that of M3 mAChR was 0.40 times higher （P=0.004）, that of M5 mAChR was 1.23 times higher （P=0.488）, that of α5 nAChR was 0.63 times higher （P=0.177）, that of α7 nAChR was 0.81 times higher （P=0.668）, and that of AChE was 3.24 times higher than that of control group （P=0.002）.Conclusion The expression of nAChR （α3, α9, β2）, M3 mAChR and AChE in PBMCs of MG patients is different from contols.

关 键 词：重症肌无力 外周血单个核细胞 非神经元型胆碱能系统 

分 类 号：R746.1[医药卫生—神经病学与精神病学]