树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化  

Changes of Th1/Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis

作　　者：李妍[1] 李丽红[2] 刘海[1] 盛智超[1] 闵婕[1] 胡竹林[1] 

机构地区：[1]昆明医科大学第四附属医院眼科、云南省第二人民医院眼科、云南省眼科研究所、云南省眼科疾病防治研究重点实验室、云南省第二人民医院白内障与眼底疾病防治省创新团队,云南省昆明市650000 [2]中国科学院昆明动物研究所动物模型与人类疾病机理重点实验室,云南省昆明市650000

出　　处：《眼科新进展》2017年第9期824-827,共4页Recent Advances in Ophthalmology

基　　金：云南省卫生科技计划项目(编号:2016NS182、2017NS129);昆明医科大学博士创新基金(编号:2016D07)~~

摘　　要：目的观察树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化,了解Th1/Th2炎性因子与本病炎症反应的关系。方法茄病镰刀菌培养7 d后收集真菌混悬液,调整孢子密度为10×109CFU·m L-1。清洁级树鼩40只随机分为实验组30只、对照组10只,右眼为实验眼。实验组将真菌孢子混悬液50μL注入角膜基质中央,对照组注入生理盐水50μL。造模后第3天、第7天、第14天,流式细胞仪分析房水中白细胞介素(interleukin,IL)-1β、IL-6、IL-4、IL-10的水平;病理学检查观察浸润细胞类型。结果 Th1型细胞因子IL-1β和IL-6浓度均在造模后第7天达到高峰,造模后各时间点与对照组相比差异均具有统计学意义(均为P<0.05);Th2细胞因子IL-10浓度在造模后第14天达到高峰,造模后第7天、第14天与对照组相比差异均具有统计学意义(均为P<0.05);IL-4浓度仅在造模后第7天与对照组相比差异有统计学意义(P<0.05)。病理学检查结果示:炎症细胞浸润数量造模后第7天达到高峰,以中性粒细胞为主;实验组各时间点均可见菌丝平行于基质纤维生长。结论促炎因子IL-1β和IL-6及抑炎因子IL-10在树鼩茄病镰刀菌性角膜炎炎症反应机制中发挥重要作用。Objective To observe the changes of Thl / Th2 inflammatory fac- tors in the aqueous humor of tree shrews with fusarium solani keratitis, as well as to explore the relationship between Thl / Th2 inflammatory factors and inflammatory re- sponse in fusarium solani keratitis. Methods Forty healthy tree shrews were ran- domly divided into experimental group（ n = 30 ） and control group （n = 10）. Fusarium solani was inoculated into sabina culture medium and cultured at 26 ℃ for 7 days, and then the fungal suspension was collected and the density of spores was adjusted to 10 × l0^9 CFU· mL^-1. In the experimental group,50 μL flmgal spore suspension was in- jected into the center of the cornea stroma, while the control group received the same amount of saline. Next the levels of cytokines including interleukin （IL）-1β, IL-6, IL-4 and IL-10 were analyzed by flow cytometry on day 3, day 7, day 14 after successful modeling, and the changes in types of infiltrating cells were observed by histopatholog- ical examination. Results The expression level of IL-1β and IL-6 （Thl type cyto- kines） was the highest on day 7, and the difference was statistically significant at each time point when compared with the control group （ all P 〈 0.05 ）. The expression level of IL-10 （Th2 type cytokines） was the highest on day 14,and the difference was statis- tically significant at each time point when compared with the control group （ all P 〈 0. 05）. The difference in IL-4 expression was statistically significant on day 7 （P 〈 0. 05 ）. In addition, histopathological examination showed that the number of infiltra- tion cell reached its peak on day 7, mainly neutrophils, and ftmgal hyphae were ob- served to be parallel to the matrix fibers at each time point. Conelusion The proin- flammatory cytokines IL-1β and IL-6 and the anti-inflammatory cytokines IL-10 may play an important role in the molecular mechanism of inflammatory response of fusari- um solani keratitis in tree shrews.

关 键 词：树鼬 茄病镰刀菌 细胞因子 

分 类 号：R772.21[医药卫生—眼科]