比较盐酸法舒地尔对不同离体动物眼房水流畅系数的影响  

Evaluation of the effect of fasudil hydrochloride on outflow facility in enucleated eyes of different animals

作　　者：刘雯婷[1] 熊毅彤[1] 张宇燕[1] 

机构地区：[1]复旦大学附属华山医院眼科,上海市200030

出　　处：《眼科新进展》2017年第9期832-835,共4页Recent Advances in Ophthalmology

摘　　要：目的评价选择性Rho激酶抑制剂盐酸法舒地尔对离体猪眼、兔眼及牛眼的房水流畅系数的影响。方法在15 mmHg(1 k Pa=7.5 mm Hg)恒压灌注条件下,先记录离体猪眼、兔眼及牛眼的基线房水流畅系数(C0),然后随机将眼球分为对照组及实验组,每组6只,对照组使用GPBS、实验组使用100μmol·L-1盐酸法舒地尔溶液继续灌注,记录实验房水流畅系数(C1),并计算房水流畅系数增加值ΔC(ΔC=C1-C0)及房水流畅系数增加百分比ΔC%(ΔC%=ΔC/C0),利用SPSS 17.0进行配对t检验及单因素方差分析。结果猪眼对照组、实验组的ΔC%分别为(17.83±3.84)%、(44.00±6.44)%;牛眼对照组、实验组的ΔC%分别为(11.67±1.17)%、(37.17±4.84)%;兔眼对照组、实验组的ΔC%分别为(15.50±2.93)%、(31.67±6.54)%,三种动物眼的实验组ΔC%较对照组均明显增加,差异均有统计学意义(均为P<0.05)。三种不同动物离体眼实验组的ΔC%之间差异无统计学意义(F=1.064,P=0.370)。结论盐酸法舒地尔可以增加不同种类离体动物眼房水流畅系数,其作用可能是通过调节小梁房水流出道的细胞及基质骨架结构,重新分布房水引流到一个更广泛的区域而发挥增加房水流畅系数的作用。Objective To evaluate the influence of a selective Rho-associated protein kinase inhibitor （fasudil hydrochloride） on outflow facility in enucleated por- cine,rabbit and bovine eyes. Methods At the constant perfusion pressure of 15 ram- Hg （ 1 kPa = 7.5 mmHg）, the baseline coefficient of outflow facility （ CO ） of the isolated porcine, rabbit and bovine eyes was recorded respectively. The enucleated porcine eyes were divided into two groups randomly （ n = 6 ）, and they were control group and exper- imental group. The same grouping method was also used-CO the ribbit and bovine eyes. The control group was subjected to GPBS perfusion, while the experimental group was treated with 100 μmol·L^-1 fasudil solution,foUowed by recording the experimental co- efficient of outflow facility （ C1 ）, as well as calculating △C （ △C = CI - CO ） and △C% （△C% = △C/CO）. Finally, the paired t test and one-way analysis of variance were per- formed using SPSS 17.0. Results As for porcine eyes, the △C% of the control group was （ 17.83 ± 3.84 ） % while the experimental group was （ 44.00 ± 6.44 ） % ; as for rabbit eyes, the △C% of the control group was （ 15.50 ± 2.93 ） % , while the experimental group was （31.67±6.54）% ;as for bone eyes,the △C% of the control group was （ 11.67±1.17）% ,while the experimental group was （37. 17±4.48 ）%. The △C% in the experimental group was significantly in- creased when compared with the control group in three animals, with significant difference （ all P 〈 0.05 ）. There was no sta- tistical difference in △% of three experimental groups among different ldnds of animals （ all P 〈 0.05 ）. Conclusion Fa- sudil can improve outflow facility in enucleated eyes of animals, and it can redistribute aqueous humor drainage to a wider area through directly regulating the cytoskeleton of cells and matrix, resulting in increased coefficient of outflow facility.

关 键 词：盐酸法舒地尔 房水流畅系数 细胞骨架 猪眼 兔眼 牛眼 

分 类 号：R775[医药卫生—眼科]