下调STYK1基因表达可抑制结直肠癌细胞生长与侵袭  

STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro

作　　者：唐佳佳[1] 叶林[1] 

机构地区：[1]重庆医科大学附属第一医院重症医学科,重庆400016

出　　处：《第三军医大学学报》2017年第17期1734-1738,共5页Journal of Third Military Medical University

摘　　要：目的探讨STYK1基因下调对结直肠癌细胞HCT-15增殖、凋亡和迁移以及MEK/ERK和PI3K/AKT信号通路的影响。方法采用基因干扰技术抑制结直肠癌细胞HCT-15中STYK1的表达,Western blot检测抑制效果,CCK-8检测STYK1表达下调对HC-T15细胞增殖的影响,流式细胞仪检测STYK1表达下调对HC-T15细胞凋亡的影响,Transwell小室实验检测STYK1表达下调对HCT-15细胞侵袭的影响,通过Western blot检测STYK1表达下调对HCT-15细胞MEK/ERK和PI3K/AKT信号通路活性的影响。结果与干扰对照组相比,STYK1表达下调明显抑制HCT-15细胞增殖活性(P<0.01)和侵袭能力(P<0.01),并诱导细胞凋亡(P<0.01);同时,STYK1表达下调明显抑制HCT-15细胞ERK1/2和AKT蛋白的磷酸水平。结论 STYK1基因干扰可以抑制结直肠癌细胞的增殖和侵袭,并诱导凋亡,其机制可能与抑制MEK/ERK和PI3K/AKT信号通路活性有关。Objective To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 （STYK1） on the proliferation, apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15. Methods The expression of STYK1 protein was down- regulated by RNA interference technique in HCT-15 cells. Then, the STYK1 protein expression was detected by Western blotting. CCK-8 assay was used to measure the effect of STYK1 down-regulation on the proliferation of the cells, flow cytometry was employed to detect the cell apoptosis, and Transwell chamber assay was adopted for cell invasion ability. The expression levels of p-ERK1/2 and p-AKT were detected by Western blotting. Results Colnpared with the siRNA NC group, STYK1 down-regulation significantly inhibited the proliferation （P 〈 0.01 ） and invasion （P 〈 0.01 ）, induced cell apoptosis （P 〈 0.01 ）, and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA （P 〈0.01）. Conclusion STYK1 down-regulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells, which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.

关 键 词：miR-671 直肠癌 增殖 迁移 

分 类 号：R394.3[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]