千金子对肾癌模型裸鼠癌症组织Livin蛋白及TNF-α表达的影响研究  

Effects of Semen Euphorbiae Lathyridis on the expression of Livin protein and TNF-α in cancer tissues of nude mouse model of renal cancer

作　　者：董建设[1] 赵俊峰[1] 陈瑞廷[1] 张林超[1] 孙继建 潘世杰[1] 杨锦建[2] 

机构地区：[1]河南省中医院(河南中医药大学第二附属医院)泌尿外科,郑州450008 [2]郑州大学第一附属医院泌尿外科,郑州450052

出　　处：《癌症进展》2017年第6期631-633,649,共4页Oncology Progress

摘　　要：目的探讨千金子对肾癌模型裸鼠癌症组织Livin蛋白水平及肿瘤坏死因子-α(TNF-α)表达的影响。方法采用皮下肾癌Renca细胞株悬液注射法建立肾癌裸鼠模型,共50只,接种当天随机分为对照组、治疗组(成瘤5 d后再予以千金子),每组25只,分别于成瘤后第5、10、15、20、25天采用ELISA法测定TNF-α水平,Western Blot分析测定癌症组织Livin蛋白表达情况,同时测定两组裸鼠肿瘤直径,并记录肿瘤生长抑制率。结果第20天开始,治疗组血清中TNF-α水平低于对照组,差异有统计学意义(P﹤0.05);从第15天开始,治疗组的癌症组织Livin蛋白水平高于对照组,差异有统计学意义(P﹤0.05);从第10天开始,治疗组的肿瘤体积小于对照组,差异有统计学意义(P﹤0.05);随着给药时间的延长,抑瘤率逐渐增高。结论千金子可以降低肾癌模型裸鼠癌症组织Livin蛋白及TNF-α表达水平,对裸鼠肿瘤增长具有抑制作用。Objective To investigate the effects of Semen Euphorbiae Lathyridis on the expression of Livin protein and tumor necrosis factor-α（TNF-α） in cancer tissues of nude mouse model of renal cancer. Method 50 nude mouse xenograft models of renal cancer were established by subcutaneously injecting renal cancer Renca cell strain suspension.These mice were randomly divided into control group and treatment group（Semen Euphorbiae Lathyridis was given 5 days after the formation of tumor） on inoculation day, with 25 mice in each group. The level of TNF-α was measured by ELISA and the expression level of Livin protein in cancer issues was analyzed by Western blot on day 5, 10, 15, 20 and 25 after tumor formation. At the same time, the tumor diameters in nude mice of the two groups were measured and the inhibition rates of tumor growth were recorded. Result Starting from day 20, the serum level of TNF-α of the treatment group was lower than that of the control group, and the difference between two groups reached statistically significant（P〈0.05）; starting from day 15, the Livin protein level in cancer issues of treatment group was significantly higher than that of the control group, and statistically significant differences were observed（P〈0.05）; and staring form day 10, the tumor volume of the treatment group was smaller than that of the control group, and the differences between two groups were statistically significant（P〈0.05）; with the prolongation of administration time, the tumor inhibitory rate gradually increased. Conclusion Semen Euphorbiae Lathyridis can significantly reduce the expression levels of TNF-α and Livin protein in cancer issues of nude mouse model of renal cancer, and can inhibit the tumor growth of nude mice.

关 键 词：千金子 肾癌模型裸鼠 LIVIN蛋白 TNF-Α 

分 类 号：R737.11[医药卫生—肿瘤]