骨髓源性内皮祖细胞对肺癌新生血管形成的作用  

The role of bone marrow-derived endothelial progenitor cells for lung cancer neovascularization

作　　者：柳瑞军[1] 熊健 陈苏峰[3] 

机构地区：[1]上海交通大学附属胸科医院肺部肿瘤临床医学中心,上海200030 [2]上海市浦东医院,上海201209 [3]复旦大学附属肿瘤医院,上海200032

出　　处：《中国胸心血管外科临床杂志》2017年第9期716-720,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金(30571843)

摘　　要：目的观察内皮祖细胞(endothelial progenitor cells,EPCs)参与肺腺癌新生血管的形成。方法利用携带Lac Z基因的重组腺病毒,以最佳转染浓度转染EPCs,然后将EPCs经肺腺癌动物模型的尾静脉注入,分别在第6、7、8周取肺组织进行病理切片,X-gal染液显色,观察EPCs参与肺癌血管的形成。结果 AD5F35Lac Z转染EPCs的最佳感染复数(multiplicity of infection,MOI)为400;当MOI=400时,AD5F35Lac Z的转染率最大,为97.13±2.08。Lac Z基因转染的EPCs体外培养2周后开始增殖,移植入肺癌动物模型后,第8周参与肺癌组织新生血管的形成。结论 EPCs移植后参与了肿瘤新生血管的形成。Objective To observe endothelial progenitor cells （EPCs） participating in the formation of neovascularization in lung adenocarcinoma. Methods EPCs were transfected by recombinant adenovirus carrying LacZ gene in optimal transfection concentration, and then EPCs were injected into animal models of lung adenocarcinoma through the tail vein; afterwards, lung tissues were taken out for pathological examination in the 6th, 7th, 8th week respectively. EPCs were observed to take part in the angiogenesis in the lung adenocarcinoma through X-gal chromogenic dye. Results The optimal multiplicity of infection （MOI） of ADSF35LacZ transfected EPCs was 400. When MOI was 400, maximum transfection efficiency was 97.13±2.08. After 2 weeks, LacZ gene-transfected EPCs began to proliferate in vitro culture, then the EPCs were transplanted into animal models of lung cancer to be involved in the neovascularization formation in the 8th week after transplantation. Conclusion EPCs are involved in the formation of tumor neovascularization after transplantation.

关 键 词：内皮祖细胞 血管再生 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]