白杨素抑制人膀胱癌裸鼠移植瘤生长的初步研究  

A pilot experimental study on growth inhibition of human bladder cancer transplanted tumor in nude mice treated with chrysin

作　　者：李卿[1] 何娅娣[2] 罗子寰[3] 陈晓波[3] 高新[3] 李辽源[3] Li Qing He Yadi Luo Zihuan Chen Xiaobo Gao Xin Li Liaoyuan(Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China Center of Physical Examination Department of Urology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.)

机构地区：[1]中山大学公共卫生学院营养系,广州510080 [2]中山大学附属第三医院体检中心,广州510630 [3]中山大学附属第三医院泌尿外科,广州510630

出　　处：《中华腔镜泌尿外科杂志（电子版）》2017年第4期53-56,共4页Chinese Journal of Endourology(Electronic Edition)

基　　金：国家自然科学基金(81502206)

摘　　要：目的探讨白杨素(Ch R)对膀胱癌T24细胞裸鼠移植瘤生长的影响。方法建立人膀胱癌T24裸鼠皮下异体移植模型(n=40)。接种4周全部成瘤后,将动物随机分为5组:对照组(n=8):腹腔内注射生理盐水,隔日给药1次;丝裂霉素(MMC)组(n=8):腹腔内注射MMC,4 mg/kg,隔日给药1次;低剂量Ch R组(L-Ch R,n=8):腹腔内注射Ch R,20 mg/kg,隔日给药1次;中剂量Ch R组(M-Ch R,n=8):腹腔内注射Ch R,40 mg/kg,隔日给药1次;高剂量Ch R组(H-Ch R,n=8):腹腔内注射Ch R,80 mg/kg,隔日给药1次。连续治疗20 d,裸鼠每周称重2次,测量肿瘤2次,治疗结束处死动物,取出肿瘤称重计算抑瘤率。结果 Ch R(20 mg/kg、40 mg/kg、80 mg/kg)对人膀胱癌T24细胞皮下移植瘤瘤重抑制率分别是40.5%、62.8%和73.4%,与对照组比较,移植瘤重量显著降低(P<0.05);不同剂量Ch R组裸鼠的白细胞计数、血清谷丙谷草转氨酶、尿素氮及肌酐与生理盐水模型对照组相比无显著性差异(P>0.05)。结论 Ch R能显著抑制人膀胱癌裸鼠移植瘤生长,呈剂量和时间依赖性,是一种毒副作用较小的治疗膀胱癌新候选药物。Objective To investigate the inhibition effect of chrysin（Ch R） on the growth of xenotransplanted tumor with human bladder cancer cell line in vivo. Methods Xenotransplanted model of a human bladder cancer cell T24 in the dorsal skin of nude mice was established. Four weeks later, 40 mice were randomly divided into 5 groups.Control group（n=8）： 0.9% NS injected intraperitoneally on alternate days; MMC group（n=8）： MMC was injected intraperitoneally at dose of 4 mg/kg on alternate days; L-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 20 mg/kg on alternate days; M-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 40 mg/kg on alternate days; H-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 80 mg/kg on alternate days. Twenty days after treatment, tumor weight, size and tumor growth inhibition rate were evaluated respectively. Results After treatment with Ch R in nude mice model, the tumor growth inhibition rate of Ch R in 20 mg/kg, 40 mg/kg, 80 mg/kg concentration was 40.5%, 62.8% and 73.4% respectively, which was significantly different compared with control group treated with NS（P〈0.05）. The changes of WBC, serum ALT, AST, BUN and creatine levels in nude mice of each Ch R group were not significantly different compared with control group treated with（P〈0.05）. Conclusion Ch R can inhibit the tumor growth of human bladder cancer line T24 cells in nude mice and show a dose-and time-dependence. It may be a new candidate for the treatment of bladder cancer with low toxicity.

关 键 词：膀胱肿瘤 白杨素 裸鼠 移植瘤 

分 类 号：R737.14[医药卫生—肿瘤]