α-突触核蛋白与安神醒脑中药有效成分的相互作用  

Interactions between α-synuclein and effective components of Anshenxingnao traditional Chinese medicine

作　　者：孔令茹[1] 刘曙玮 邓月义[1] Kong Lin- gru Liu Shuwei Deng Yueyi(College of Pharmacy, Guilin Medical University, Guilin, Guangxi 541004 China)

机构地区：[1]桂林医学院药学院,广西桂林541004

出　　处：《现代医药卫生》2017年第17期2588-2590,2595,共4页Journal of Modern Medicine & Health

基　　金：广西自然科学基金资助项目(2014GXNSFBA118052);广西高校科研资助项目(XM20141209173713259)

摘　　要：目的研究α-突触核蛋白与安神醒脑中药(如葛根素、大黄酚、熊去氧胆酸)有效成分的相互作用,考察药物与α-突触核蛋白之间的结合状态。方法通过荧光滴定法研究α-突触核蛋白与安神醒脑中药的相互作用,利用scatchard方程对荧光数据进行分析,获得α-突触核蛋白-药物复合物的解离常数(KD)。结果分别加入葛根素、大黄酚、熊去氧胆酸后,α-突触核蛋白的荧光强度均逐渐降低,发射峰位置未发生位移。反之葛根素、大黄酚、熊去氧胆酸的荧光强度在加入α-突触核蛋白后逐渐升高。通过蛋白质荧光数据获得的葛根素、大黄酚、熊去氧胆酸与α-突触核蛋白复合物KD值分别为0.368 5×10-5、2.778 2×10-5、0.317 2×10-5 mol/L,通过药物荧光数据获得的KD值与其基本一致。结论葛根素、大黄酚、熊去氧胆酸分别与α-突触核蛋白存在结合作用,从而猝灭α-突触核蛋白的荧光,其中熊去氧胆酸与α-突触核蛋白的结合作用较强。Objective To study the interactions between α-synuclein and the effective components（puerarin,chrysophanol,ursodesoxycholic acid） of Anshenxingnao traditional Chinese medicines and to investigate the junction state of medica-tions with α-synuclein. Methods The interactions between α-synuclein and the effective components of Anshenxingnao traditional Chinese medicines was studied by using the fluorescence titrimetric method. The fluorescence data were analyzed by using the Scatchard equation for obtaining the dissociation canstant KDof α-synuclein-drug complexes. Results After adding puerarin,chrysophanol and ursodeoxycholic acid respectively,the fluorescent strength of α-synuclein was gradually reduced and the emission peak location had no shift. On the contrary,the fluorescence strengthes of puerarin,chrysophanol and ursodeoxycholic acid were gradually enhanced after adding the α-synuclein. The KD values of puerarin,chrysophanol,ursodeoxycholic acid and α-synuclein by protein fluorescence data were 0.368 5×10^-5,2.778 2×10^-5,0.317 2×10^-5mol/L respectively. The KD values obtained by drug fluorescence data were basically consistent with them. Conclusion The binding action respectively exists between puerarin,chrysophanol and ursodesoxycholic acid with α-synuclein,thus which quenches the fluorescence of α-synuclein,in which the binding action between ursodeoxycholic acid and α-synuclein is stronger.

关 键 词：突触蛋白类 葛根素 大黄酚 熊脱氧胆酸 中药化学成份 荧光光度测定法 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学]