Extraction of Perilla Seed Oil Using Supercritical Carbon Dioxide and GC-MS Analysis  

正交试验法优化超临界CO_2萃取紫苏籽油的工艺及GC-MS成分分析(英文)

作　　者：杨丽嫔 周游[1] 耿姗姗[1] 刘末[1] 雷红[1] 

机构地区：[1]南京财经大学食品科学与工程学院,江苏南京210023

出　　处：《Agricultural Science & Technology》2017年第8期1509-1512,共4页农业科学与技术（英文版）

基　　金：Supported by Undergraduate Innovation Training Program of Jiangsu Province(201610327010Z)~~

摘　　要：[Objective] This study was aimed to determine the optimal parameters for the extraction of perilla seed oil to obtain high-quality perilla seed oil and analyze its compositions. [Method] In this study, perilla seed oil was extracted using supercritical CO2 （SC-CO2）. The effects of extraction time, temperature and pressure were investigated by single-factor experiments and orthogonal array testing （ORT）. The chemical compositions of extracted perilla seed oil were investigated by GC-MS. [Result] The optimal conditions for the extraction of perilla seed oil using SC-CO2 were extraction time of 4 h, extraction temperature at 40 ℃, and extraction pressure at 23 MPa. Under these conditions, the extraction yield of perilla seed oil was maximized to 12.43%. GC-MS analysis revealed that perilla seed oil was a complex mixture containing 76.183% α-linolenic acid. [Conclusion] Supercritical CO2 extraction was proven to be an effective technology to extract oil from perilla seed, and GCMS was also a satisfactory method for analyzing the compositions of perilla seed oil.[目的]优化超临界CO_2法萃取紫苏籽油的条件,并分析紫苏籽油的化学成分。[方法]采用单因素和正交试验法优化萃取时间、萃取温度和萃取压强,用GC-MS分析紫苏籽油的化学成分组成。[结果]通过单因素和正交试验结果分析得到,最优紫苏籽油萃取条件为:萃取时间4h,萃取温度40℃,萃取压强23 MPa,在此条件下的得油率为12.43%。用GC-MS分析紫苏籽油化学成分,结果表明α-亚麻酸的含量达到76.183%。[结论]在试验所得条件下,可获得高质量和高纯度的紫苏籽油。

关 键 词：Perilla seed essential oil Supercritical carbon dioxide extraction Or-thogonal array testing （OAT） GC-MS 

分 类 号：O657.63[理学—分析化学] TS225.1[理学—化学]