miR-148a在膀胱癌中的异常表达及临床意义  

The abnormal expression of miR-148a in bladder cancer and its clinical significance

作　　者：茅利明 陈玉华[2] 廖昭平 

机构地区：[1]中国医大绍兴医院,浙江绍兴312030 [2]浙江大学医学院附属第二医院,浙江杭州310009

出　　处：《中国卫生检验杂志》2017年第15期2143-2145,2161,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的比较miR-148a在膀胱癌(BCa)组及正常对照组中的相对表达水平,并进行临床意义的初步探讨。方法2011年-2013年采集于浙江大学医学院附属第二医院泌尿外科诊疗中心BCa患者标本52例为膀胱癌组,同时选正常对照组24例,用RT-PCR对产物进行反转录反应和扩增,分析miR-148a在膀胱癌组和正常对照组结果之间的差异并做比较。结果膀胱癌组标本中miR-148a的表达水平明显高于正常对照组,差异有统计学意义(Z=-6.358,P<0.05);BCa组患者病理分级越高,miR-148a在癌组织中的表达升高越明显,差异有统计学意义(χ~2=22.163,P<0.05);Ⅱ级与Ⅲ级、Ⅰ级与Ⅲ级之间比较差异均有统计学意义(Z值分别为-3.447、-3.67,P<0.05)。膀胱癌伴肿瘤浸润组(T2)的miR-148a表达明显高于非浸润组(Tis原位癌+T1),差异有统计学意义(Z=-3.37,P<0.05)。结论miR-148a可能在BCa的病理分级和临床分期中有着一定的作用,为miR-148a在BCa肿瘤形成及肿瘤治疗方面提供了新的视点。Objective To compare the relative expression levels of miR-148 a in bladder cancer（ BCa） group and normal control group,and explore the clinical significance. Methods From 2011 to 2013,52 samples of human BCa tissues were collected from the Second Affiliated Hospital of Medicine College of Zhejiang University as BCa group,and 24 normal samples were collected as control group. The reverse transcription reaction and amplification process were done by RT-PCR. miR-148 a difference between BCa group and control group was compared. Results The expression of miR-148 a in BCa group was obviously higher than that in normal group,and the difference was statistically significant（ Z =-6. 358,P〈0. 05）. The higher the pathological grade of BCa group,the higher the expression of miR-148 a in cancer tissues,the difference was statistically significant（ χ~2= 22. 163,P〈0. 05）. There was statistical significance on differences between stage Ⅱ and stage Ⅲ,stage Ⅰand stage Ⅲ（ Z =-3. 447,Z =-3. 67,respectively,P〈0. 05）. The expression of miR-148 a in the BCa group with the tumor infiltrating（ T2） was significantly higher than that in the non invasive group（ Tis in situ carcinoma ＋ T1）,and the difference was statistically significant（ Z =-3. 37,P〈0. 05）. Conclusion miR-148 a may play an important role in BCa tumor progression and clinical stage. It provides a new viewpoint in bladder tumorigenesis and therapy.

关 键 词：膀胱癌 实时荧光定量PCR miR-148a 微小RNA 

分 类 号：R737.14[医药卫生—肿瘤]