2-甲氧基雌二醇联用抗坏血酸诱导白血病K562细胞凋亡机制研究  

Study on apoptosis of leukemia K562 cells induced by 2-methoxyestradiol combined with ascorbic acid and its mechanism

作　　者：张苏伟[1] 曾嫦[1] 林祥伟[1] 

机构地区：[1]汕头市中心医院,广东汕头515000

出　　处：《中国校医》2017年第8期609-614,共6页Chinese Journal of School Doctor

基　　金：汕头市科技计划项目(2015 123-6)

摘　　要：目的研究2-ME联合抗坏血酸在诱导白血病细胞凋亡中的作用以及相关作用机制。方法用显微镜观察K562细胞凋亡情况;通过流式细胞术分析凋亡率;通过EMSA检测NF-κB的活性。结果 2μmol/L2-ME+62.5μmol/LAA组可诱导K562细胞凋亡。2-ME+AA以剂量依赖性方式诱导凋亡,在浓度(2~8μmol/L)时凋亡呈时间-剂量依赖。2-ME可诱导NF-κBDNA结合活性以剂量依赖性方式降低。结论 2-ME联合抗坏血酸可诱导白血病细胞以时间-剂量依赖式凋亡,其诱导凋亡的机制与NF-κB通路有关。Objective To investigate the role of 2-methoxyestradiol combined with ascorbic acid in the apoptosis of leukemia K562 cells and its related molecular mechanisms. Methods The apoptosis of K562 cells was observed by a microscope. The apoptosis rate was analyzed by flow cytometry. The activity of NF-KB was detected by EMSA. Results The apoptosis of K562 cells was induced by 2 μmol/L 2-ME ＋ 62.5 μmol/LAA. 2-ME ＋ AA induced apoptosis in a dose- dependent manner. The apoptosis was time-dose dependent at the concentration （2-8 μmol / L）. 2-ME could induce NF-KB DNA binding activity in a dose-dependent manner. Conclusion 2-methoxyestradiol combined with ascorbic acid can induce the apoptosis of leukemia K562 cells in a time-dose-dependent manner. The mechanism is related to the pathway of NF-KB.

关 键 词：白血病 髓内 慢性 BCR-ABL阳性/治疗 K562细胞 细胞凋亡/药物作用 NF-κB/拮抗剂和抑制剂 2-甲氧雌二醇 谷胱甘肽 抗坏血酸 电泳迁移位移实验/方法 流式细胞术/方法 

分 类 号：R446.1[医药卫生—诊断学]