ICAM-1、VCAM-1和I-FABP在狼疮肠病小鼠中表达的研究  

Expressions of ICAM-1,VCAM-1 and I-FABP in Lupus Enteropathy in Mice

作　　者：霍静[1] 罗雯[1] 李明玮[1] 王佩佩[1] 

机构地区：[1]首都医科大学附属复兴医院风湿免疫科,100038

出　　处：《胃肠病学》2017年第8期456-459,共4页Chinese Journal of Gastroenterology

基　　金：北京市教委科技计划面上项目(KM201410025020)资助

摘　　要：背景:系统性红斑狼疮并发狼疮肠病的预后差,目前有关狼疮肠病的发生机制研究甚少。目的:探讨细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、肠脂肪酸结合蛋白(I-FABP)在狼疮肠病小鼠中的表达。方法:将20只MRL/lpr自发性狼疮小鼠随机分为狼疮肠病组和对照组。以TNBS灌肠诱导狼疮肠病模型。行组织学评分,以免疫组化染色检测ICAM-1、VCAM-1、I-FABP表达,并分析其与组织学评分的相关性。结果:与对照组相比,狼疮肠病组结肠组织学评分显著升高(8.1±5.8对0.8±0.5,P=0.000),ICAM-1(9.4%±2.1%对6.2%±1.1%)、VCAM-1(15.1%±2.1%对12.2%±1.9%)、I-FABP(17.5%±2.5%对6.1%±0.9%)表达均显著升高(P<0.05)。狼疮肠病组结肠组织ICAM-1、VCAM-1和I-FABP表达与组织学评分呈正相关(r=0.870,P=0.010;r=0.881,P=0.010;r=1.000,P=0.000)。结论:ICAM-1、VCAM-1和I-FABP可能与狼疮肠病的发病相关。Background：The prognosis of systemic lupus erythematosus complicated with lupus enteropathy is poor.At present,studies on pathogenesis of lupus enteropathy are rare.Aims：To investigate the expressions of intercellular adhesion molecule-1（ICAM-1）,vascular cell adhesion molecule-1（VCAM-1）and intestinal fatty acid binding protein（I-FABP）in lupus enteropathy in mice.Methods：Twenty MRL/lpr lupus mice were randomly divided into two groups：lupus enteropathy group and control group.Lupus enteropathy model was established by administration with TNBS enema.Histological score was assessed,expressions of ICAM-1,VCAM-1 and I-FABP were determined by immunohistochemistry,and correlations with histological score were analyzed.Results：Compared with control group,histological score was significantly increased（8.1±5.8 vs.0.8±0.5,P=0.000）,expressions of ICAM-1（9.4%±2.1%vs.6.2%±1.1%）,VCAM-1（15.1%±2.1%vs.12.2%±1.9%）and I-FABP（17.5%±2.5%vs.6.1%±0.9%）were significantly increased（P〈0.05）in lupus enteropathy group.Expressions of ICAM-1,VCAM-1 and I-FABP in colon tissue in lupus enteropathy group were positively correlated with histological score（r=0.870,P=0.010;r=0.881,P=0.010;r=1.000,P=0.000）.Conclusions：ICAM-1,VCAM-1 and I-FABP may be associated with pathogenesis of lupus enteropathy.

关 键 词：红斑狼疮 系统性 狼疮肠病 细胞黏附分子 血管细胞黏附分子1 脂肪酸结合蛋白质类 

分 类 号：R574[医药卫生—消化系统] R593.241[医药卫生—内科学]