普萘洛尔及异丙肾上腺素对婴儿血管瘤内皮细胞β2肾上腺素受体表达的影响  

In vitro effects of propranoloi and isoproterenol on the expression of beta-2 adrenergic receptor on infantile hemangioma endothelial cells

作　　者：朱雅琳[1] 马龙[1] 帕丽达·阿布利孜[1] 

机构地区：[1]新疆医科大学第一附属医院皮肤科,乌鲁木齐830054

出　　处：《中华皮肤科杂志》2017年第9期673-675,共3页Chinese Journal of Dermatology

摘　　要：目的 探讨β2肾上腺素受体在婴儿血管瘤发病机制中的作用。方法 婴儿血管瘤内皮细胞分为普萘洛尔组及异丙肾上腺素组。普萘洛尔组再分为10、15、20 μg/ml组、空白组及二甲基亚砜（DMSO）组5组，异丙肾上腺素组再分为5、10、20 μg/ml组、空白组4组。血管瘤内皮细胞与这些组作用24 h、48 h，用酶联免疫吸附法分别测定每浓度细胞培养上清液中β2肾上腺素受体的表达。结果 作用24 h后，15 μg/ml、20 μg/ml组普萘洛尔与空白组和DMSO组比较，β2肾上腺素受体的表达下降，差异有统计学意义（均P＜0.05）。48 h后，普萘洛尔组各浓度培养的细胞，β2肾上腺素受体表达较空白组下降，差异有统计学意义（均P＜0.05）。而异丙肾上腺素组，作用24 h后，10 μg/ml、20 μg/ml组的异丙肾上腺素较空白组β2肾上腺素受体的表达增高，差异有统计学意义（均P＜0.05）。48 h后，20 μg/ml组异丙肾上腺素培养的细胞上清液中的β2肾上腺素受体表达较空白组高，差异有统计学意义（均P＜0.05）。结论 普萘洛尔对血管内皮细胞表面的β2肾上腺素受体表达的影响下调，而异丙肾上腺素作用上调。Objective To explore the role of beta-2 adrenergic receptor in the pathogenesis of infantile hemangioma. Methods In vitro cultured infantile hemangioma endothelial cells were divided into propranolol and isoproterenol groups. The propranolol groups were further classified into 5 groups to be treated with propranolol solutions at concentrations of 10, 15, 20 μg/ml, EGM-2 medium （blank control group 1）, and EGM-2 medium containing 0.16% DMSO （DMSO group） respectively, while the isoproterenol groups were classified into 4 groups to be treated with isoproterenol solutions at concentrations of 5, 10, 20 μg/ml and EGM-2 medium （blank control group 2） respectively. After 24- and 48-hour treatment, enzyme-linked immunosorbent assay （ELISA） was performed to measure the of beta-2 adrenergic receptor in cell culture supernatants in the above groups. Results After 24-hour treatment, 15-μg/ml and 20-μg/ml propranolol groups showed significantly decreased of beta-2 adrenergic receptor compared with blank control group 1 and DMSO group （all P 〈 0.05）. After 48-hour treatment, all the propranolol groups showed significantly decreased of beta-2 adrenergic receptor compared with the blank control group 1（all P 〈 0.05）. However, the of beta-2 adrenergic receptor was significantly higher in the 10- and 20-μg/ml isoproterenol groups than in the blank control group 2 after 24-hour treatment （all P 〈 0.05）, and higher in the 20-μg/ml isoproterenol group than in the blank control group 2 after 48-hour treatment （P 〈 0.05）. Conclusion Propranolol can down-regulate the of beta-2 adrenergic receptor on the surface of vascular endothelial cells, while isoproterenol can up-regulate its .

关 键 词：血管瘤 婴儿 内皮细胞 普萘洛尔 异丙肾上腺素 受体 肾上腺素能β2 

分 类 号：R732.2[医药卫生—肿瘤]