全身低能量光疗对重度烧伤大鼠早期全身炎症反应的影响  

Effect of systemic low-level light therapy on early systemic inflammatory response of severe burn rats

作　　者：金剑[1] 何放[1] 罗鹏飞[1] 胡晓燕[1] 夏照帆[1] 

机构地区：[1]第二军医大学长海医院烧伤科,上海200433

出　　处：《第二军医大学学报》2017年第8期987-992,共6页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81401596);Supported by National Natural Science Foundation of China(81401596);上海市自然科学基金(13ZR1408800);Natural Science Foundation of Shanghai(13ZR1408800);第二军医大学长海医院"1255"学科建设计划(CH125540600);the"1255"Discipline Construction Project of Changhai Hospital of Second Military Medical University(CH125540600)

摘　　要：目的探讨全身低能量光疗(LLLT)对重度烧伤大鼠全身炎症反应的影响。方法将50只SD大鼠随机分为空白对照组、单纯烧伤组、烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组,每组10只。单次短时光照组、烧伤后单次长时光照组、烧伤后多次短时光照组大鼠在烧伤后均给予低强度LED光(640nm)照射全身,分别照射5min/d、15min/d、5min×3次/d。各组大鼠分别于造模前1d,造模后即刻,造模后第1、3、7、14天取尾静脉血检测血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6的含量,计数静脉血白细胞;并于造模后第3、7、14天测量创面面积,计算创面愈合率。结果与空白对照组相比,造模后第1天时单纯烧伤组及烧伤后单次短时光照组大鼠血清TNF-α含量升高(P<0.05),而烧伤后单次长时光照组及烧伤后多次短时光照组分别于造模后第1、7天时升高(P<0.05);各组大鼠血清IL-1β含量均在造模后第1天时下降(P<0.05),后逐渐恢复,但恢复速度不一;单纯烧伤组及烧伤后多次短时光照组大鼠血清IL-6含量均在造模后第1天时升高(P<0.05),后下降,但单纯烧伤组下降幅度大于烧伤后多次短时光照组,而烧伤后单次短时光照组于造模后第3天升高后下降,烧伤后单次长时光照组呈持续升高态势(P<0.05)。单纯烧伤组及烧伤后单次长时光照组大鼠静脉血白细胞计数均于造模后第1天时升高并维持(P<0.05),其余各组白细胞计数无明显改变。烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组大鼠创面愈合率均高于单纯烧伤组(P<0.05)。结论全身LLLT可改善严重烧伤大鼠血清炎症因子TNF-α、IL-1β、IL-6的含量及静脉血白细胞计数,促进创面愈合,且不同照射模式所产生的效果不一。Objective To explore the effect of systemic low-level light therapy（LLLT）on early inflammatory response of severe burn rats.Methods Fifty SD rats were randomly divided into control group,burned model group,single short-term LLLT group,single long-term LLLT group and the repeated short-term LLLT group,with 10 rats in each group.After burning the rats in the single short-term LLLT group,the single long-term LLLT group and the repeated short-term LLLT group were treated by low-intensity LED irradiation（640nm）for 5min once a day,15 min once a day and 5min three times a day,respectively.The levels of tumor necrosis factorα（TNF-α）,interleukin（IL）-1βand IL-6and the leukocyte count in caudal vein were determined at 1day before modeling,immediately after modeling and on the 1st,3rd,7th and 14 th day after modeling;and the wound area was measured on the 3rd,7th and 14 th day after modeling.The wound healing rate was calculated.Results Compared with the control group,the serum TNF-αlevels in the burned model and single short-term LLLT groups were significantly increased on the 1st day after modeling（P〈0.05）,and the serum TNF-αlevels in the single long-term LLLT group on the 1st day and the repeated short-term LLLT group on the 7th day were significantly increased（P〈0.05）;the serum IL-1βlevels were significantly decreased on the1 st day after modeling in all groups（P〈0.05）,and then gradually recovered with the varied recovery rates;the serum IL-6levels in the burned model and the repeated short-term LLLT groups were significantly increased on the 1st day after modeling（P〈0.05）,then decreased;and the decrease of the burned model group was greater than that of the repeated short-term LLLT group.While the serum IL-6level was increased on the 3rd day in the single short-term LLLT group,then decreased;and the level was significantly increased in the single long-term LLLT group（P〈0.05）.Leukocyte counts of the burned model and the single long-term LLLT groups were significantly in

关 键 词：光疗法 烧伤 全身炎症反应 肿瘤坏死因子α 白介素1Β 白介素6 白细胞 创面愈合率 

分 类 号：R644[医药卫生—外科学]