斑马鱼模型在糖尿病研究中的应用  

Application of zebrafish models in research of diabetes

作　　者：王雪[1] 韩利文[1] 何秋霞[1] 韩建[1] 王荣春[1] 陈维云[1] 王希敏[1] 侯海荣[1] 刘可春[1] 

机构地区：[1]山东省科学院生物研究所,山东省生物检测技术工程实验室,山东省科学院药物筛选技术重点实验室,济南250014

出　　处：《中国比较医学杂志》2017年第8期1-5,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(31400979);山东省自主创新重大专项(2014ZZCX0215)

摘　　要：作为一种模式生物,斑马鱼具有其他动物模型所不具备的诸多优势,非常适用于进行人类疾病建模及机制研究。斑马鱼胰腺经历两次发育分化过程,其间伴有多种信号途径的活化参与。成鱼的胰腺结构与其他动物胰腺相似,能分泌包括胰岛素在内的多种激素。斑马鱼胰腺及对胰岛素敏感的肝脏、肌肉等这些与代谢调控相关的主要器官及外周组织,在进化上具有保守性,糖代谢调控机制也与其他哺乳动物相似,这使得斑马鱼非常适用于糖代谢调控研究。除利用STZ诱导胰岛β细胞凋亡造成斑马鱼糖尿病模型外,高糖溶液交替暴露及外源性干预代谢相关信号途径也可以造成斑马鱼血糖升高。成鱼血糖水平、幼鱼组织糖含量、视网膜及视网膜血管形态变化是目前斑马鱼糖尿病模型研究中的常用检测指标。As a model organism,zebrafish have many advantages over other animal models and is suitable for studies on establishment of human disease model and mechanism. In zebrafish,there are two phases of endocrine formation during early development,which are directed by concomitant activity of many signaling pathways. Zebrafish pancreas possess similar cell structure with that of other animals,which can express various endocrine hormones including insulin.The main organs required for metabolic control,such as the pancreas,islet,and insulin sensitive tissue（ muscle,liver）are conserved in zebrafish,and the mechanisms of glucose regulation in zebrafish is similar to that seen in mammalian models. These render it an excellent model to study glucose metabolism. Hyperglycemia in zebrafish model can be induced by administration of the diabetogenic drug,streptozotocin（ STZ）,alternatively immersion of the fish in glucose solution and water,or disturbing of signaling pathways associated with glucose metabolism. Glucose levels in adult zebrafish blood or embryo tissue and phenotype of retinal cell layers or retinal vasculature are the commonly used measurement organs in zebrafish diabetic models.

关 键 词：斑马鱼 糖尿病 模式生物 胰腺 糖代谢 

分 类 号：R-33[医药卫生]