两种2型糖尿病小鼠溃疡创面愈合比较  

Comparison of wound healing in two mouse models of type 2 diabetes

作　　者：韩延忠 王玉芝[2] 刘如华[3] 沈娟[1,3] 

机构地区：[1]广东药科大学基础学院药用生物活性物质研究所,广州510006 [2]广州军区广州总医院,广州510006 [3]广东药科大学生命科学与生物制药学院,广州510006

出　　处：《中国比较医学杂志》2017年第8期6-11,93,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(81502520)

摘　　要：目的比较目前常用的基因自发突变2型糖尿病小鼠C57BL/6J-db/db和STZ诱导的C57BL/6J小鼠的溃疡创面愈合特征,为应用稳定的糖尿病小鼠溃疡模型进行相关动物实验研究提供依据。方法建立糖尿病小鼠溃疡模型,统计0、3、5、7、10、14 d小鼠创面动态愈合率及愈合时间;7、14 d取组织,HE染色和Masson染色,免疫组化(CD31和PCNA)观察创面病理组织变化;荧光定量测定愈合相关因子collagen-IIIα、α-SMA的基因表达。结果基因突变db/db小鼠较STZ诱导小鼠的创面愈合时间显著延迟,由(16.6±0.8)d延长至(20.2±1.3)d(P<0.001);db/db组较STZ组肉芽组织生长缓慢,新生上皮长度不足、胶原沉积紊乱,创面愈合较差;7 d,db/db组的CD31和PCNA显著性低表达(P<0.01);7、14 d,db/db组基因上调量的倍数明显低于STZ组。结论两种糖尿病小鼠创面均出现愈合延迟,但基因突变糖尿病db/db小鼠相比于STZ诱导组小鼠在创面愈合延迟的程度上和愈合难易程度上,更适合进行糖尿病溃疡创面的研究。Objective To compare the characteristics of ulcer wound healing in current commonly used C57BL/6J-db/db mouse models of spontaneous gene mutation-induced type 2 diabetes and in C57BL/6J mice with diabetes induced by streptozotocin（ STZ）,and to provide a basis for related experimental studies on diabetic ulcer in animal models.Methods To establish the mouse models of diabetic ulcer wound,observe the healing time and calculate the wound healing rate at 0,3,5,7,10,14 days. Tissue samples were collected at days 7 and 14. HE and Masson staining,and immunohistochemistry（ CD31 and PCNA） were used to observe the pathological changes of the wound tissues. Gene expressions of collagen-IIIα,fibronectin and α-SMA were detected by fluorescent quantitative analysis. Results The wound healing time of db/db mice was significantly delayed compared with the STZ mice,which was extended from 16. 6 ±0. 8 d to 20. 2 ± 1. 3 d（ P〈0. 001）. Compared with the STZ group,the growth of granulation tissue in the db/db group was slow,the length of newly formed epithelium was insufficient,the collagen deposition was disordered,and the wound healing was poor. At 7 days,the expression of CD31 and PCNA was significantly lower in the db/db group（ P〈0. 01）, and at 7 and 14 days,the increase of collagen-IIIαandα-SMA genes up-regulation was significantly lower in the db/db group than in the STZ group.Conclusions Both the two types of diabetic mice show delayed wound healing.However,compared with the STZ-induced diabetic mice,the gene mutation db/db mice are more suitable for studies of diabetic ulcer wound healing as regarding the extent of the delay and the degree of difficulty of wound healing.

关 键 词：2型糖尿病小鼠 难愈性创面 动物模型 

分 类 号：R-33[医药卫生]