PKH26和Hoechst 33258联合示踪Uncv无毛小鼠iRhom2及其突变体蛋白  

PKH26 combined with Hoechst 33258 to trace the iRhom2 gene and its mutant proteins of Uncv mice in Vero cells

作　　者：游颖[1] 陈丙波[1] 曾林[2] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]军事医学科学院实验动物中心,北京100071

出　　处：《中国比较医学杂志》2017年第8期40-42,共3页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金重点项目(31030058);国家科技支撑计划(2011BA115B03)

摘　　要：目的利用PKH26和Hoechst 33258联合示踪Uncv无毛小鼠iRhom2及其突变体蛋白在Vero细胞中的定位。方法用PKH26对细胞膜进行染色,同时用Hoechst 33258染料对细胞核进行染色,置于激光共聚焦显微镜下观察。结果通过激光共聚焦荧光显微镜观察目的蛋白,发现野生型iRhom2分布在细胞的胞浆,而iRhom2mut于细胞浆内和细胞核内都存在。结论亚细胞定位的不同推测到iRhom2的N末端缺失突变可能对其细胞内的定位有影响。Objective To determine the localization of iRhom2 and its mutant proteins of Uncv mice in Vero cells by PKH26 combined with Hoechst 33258 staining. Methods The cell membrane was stained with PKH26,and the nuclei were stained with Hoechst 33258 dye,and observed by laser scanning confocal microscopy. Results It was found that wild iRhom2 was distributed in the cytoplasm,and its iRhom2 mutwas present both in cytoplasm and cell nuclei. Conclusions The results of our study suggest that a deletion in N-terminal of iRhom2 affects its subcellular localization.

关 键 词：PKH26 Hoechst 33258 iRhom2 定位 

分 类 号：R-33[医药卫生]