微卫星DNA分析国内24个近交系小鼠遗传状况  

Genetic quality analysis of 24 domestic inbred mouse strains by microsatellite DNA

作　　者：李银银[1] 吴绍亮 王洪[3] 萧晓琴 张双悦[4] 郭萌[1] 李长龙[4] 吕建祎[4] 刘欣[4] 陈振文[4] 杜小燕[1] 

机构地区：[1]首都医科大学基础医学院实验动物学系,北京100069 [2]山东省临沭县人民医院,山东临沂276700 [3]中国食品药品检定研究院实验动物资源研究所,北京100050 [4]首都医科大学基础医学院遗传学系,北京100069

出　　处：《中国比较医学杂志》2017年第8期43-49,共7页Chinese Journal of Comparative Medicine

基　　金：国家科技支撑计划(2015BAI07B01);国家自然科学基金(31372272;31572341)

摘　　要：目的利用微卫星位点对国内24种近交系小鼠进行遗传状况分析。方法用前期筛选的富含多态性和等位基因数多的30个小鼠微卫星位点,合成荧光标记引物,对近交系小鼠基因组DNA进行PCR的扩增,经STR扫描对各近交系小鼠品系进行基因分型。结果在24个近交系小鼠品系中,有16个品系在品系内在30个位点上均具有相同基因型。而在不同品系间同一位点具有多态性,可初步对不同品系进行鉴别区分。其余品系内个别动物存在多态性。结论所选位点可以参考用于近交系小鼠遗传质量检测分析,并进行初步品系检测鉴定。Objective To analyze the genetic quality of 24 domestic inbred strains mice using microsatellite loci panel. Methods Previously selected 30 microsatellite loci of mouse with high polymorphism and more allele numbers were used to synthesize corresponding fluorescently-labeled primers. Then the genomic DNA samples of each mouse were amplified by PCR and the products were analyzed by STR scanning to genotype the inbred strains of mice. Results Out of the 24 inbred strains,15 inbred strains showed the same genotype within one strain at 30 loci. Among different strains,microsatellite loci indicated polymorphism which could be used to distinguish different strains. However,the rest 9 strains demonstrated polymorphism within strains. Conclusions Our stuoly provides a useful microsatellite panel to detect genetic quality of inbred mice and distinguish different strains with the optimized microsatellite loci.

关 键 词：近交系小鼠 遗传质量分析 微卫星DNA 

分 类 号：R-33[医药卫生]