大豆转录因子GmWRI1基因的分离及其转录激活特性分析  

Isolation and transcriptional activation analysis of GmWRI1 transcription factor from soybean

作　　者：陈欢[1] 邓文波[1] 张莹[1] 刘伟灿[1] 于潇潇[1] 王法微[1] 王野[1] 庄帅兵 张永红[1] 王真慧[1] 李海燕[1] 

机构地区：[1]吉林农业大学生命科学院,吉林长春130118

出　　处：《中国油料作物学报》2017年第4期447-453,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31401403);吉林省科技发展计划(20140520164JH)

摘　　要：为探讨大豆转录因子GmWRI1在大豆种子油脂形成过程中的调控作用,本研究从大豆吉育72花后35d的种子中克隆得到GmWRI1全长cDNA序列,并对该基因编码的蛋白的理化性质、结构功能进行了初步分析;通过实时荧光定量PCR对花后不同时期籽粒中GmWRI1进行定量分析并对种子进行油脂含量的测定,推测GmWRI1的表达量可能与大豆籽粒中油脂含量有关。此外,利用重组DNA技术构建酵母表达载体pGBKT7-GmWRI1并转入AH109酵母菌株中,结果表明GmWRI1具有转录激活活性,属于转录激活子。To explore the role of GmWRI1 transcription factors in regulating oil development in soybean,GmWRI1 gene was cloned from seeds of Jiyu 72 at the 35 th day after flowering,and its deduced protein＇properties,structure and function were preliminary analyzed. To characterize the expression pattern of GmWRI1 at different stages after flowering,seeds of Jiyu 72 were also detected by real-time quantitative PCR. The results showed that the expression level of GmWRI1 may correlate with the soybean seeds oil content. Furthermore,p GBKT7-GmWRI1 as expression vector was constructed using recombinant DNA technology and expressed in yeast（AH109） to analyze its transcriptional activation ability. The results showed that GmWRI1 had transcriptional activation ability in yeast,which suggested that it was a transcription activator.

关 键 词：大豆 转录因子 GmWRI1 油脂含量 

分 类 号：S565.103[农业科学—作物学]