脑源性神经生长因子对mir124表达及兴奋性电流的影响  

The effect of BDNF on levels of miRNA-124 and sEPSCs

作　　者：王凤军[1] 王洪朋 单晶丽 邵正凯[1] 姜杨薇[1] 陈元元[1] 宋婷婷[1] 邱丽婷[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,黑龙江哈尔滨150001 [2]牡丹江市妇女儿童医院,黑龙江牡丹江157000 [3]哈尔滨市第二医院,黑龙江哈尔滨150056

出　　处：《中风与神经疾病杂志》2017年第8期718-720,共3页Journal of Apoplexy and Nervous Diseases

基　　金：黑龙江省教育厅面上项目(No.12541509)

摘　　要：目的探讨BDNF对海马miRNA-124表达及齿状回颗粒细胞兴奋性突触后电流(s EPSCs)的影响,以明确BDNF对颞叶内侧癫痫(MTLE)发病机制的影响。方法选取哈尔滨医科大学附属第一临床医院神经外科2008年4月-2010年10月手术治疗的MTLE患者12例。RT-pcr技术检测BDNF孵育后MTLE患者海马组织mir-124表达、膜片钳技术检测BDNF对海马颗粒细胞s EPSCs的影响。结果 (1)BDNF降低了颞叶癫痫海马miRNA-124的表达(P<0.01);(2)BDNF增加了颗粒细胞sEPSCs的频率和幅度(P<0.01)。结论 BDNF降低了颞叶癫痫海马mir-124的表达,提高颗粒细胞sEPSCs的频率和幅度,从而可能对人类MTLE的发病起到促进作用。Objective To study the effect of brain derived neurotrophic factor（ BDNF） in the pathogenesis from patients with MTLE. Methods The study was performed between April 2008 and October 2010. Surgically removed specimens were collected from the patients with MTLE. All patients gave written informed consent for research use of the biopsy materials. Surgically resected hippocampal were collected and immediately immersed in oxygenated ice-cold SACSF. The expression of Mir-124 was detected by RT-PCR and sEPSCs by patch-clamp. Results Expression of mir-124 was significantly decreased in Dentate Gyrus of MTLE compared with that of controls（ P〈 0. 01）. BDNF increases frequency and amplitude of s EPSC（ P 〈0. 01）. Conclusions The results indicate that BDNF significantly decreased the expression of mir-124 as in Dentate Gyrus from patients with MTLE. Our electrophysiological findings indicate that BDNF increase excitability on dentate granule cells. In conclusion,our work supports a role of BDNF in pathophisyology of MTLE and raises the possibility that interference with BDNF action may be a therapeutic method for patients of MTLE.

关 键 词：脑源性神经生长因子 颞叶内侧癫痫 miRNA-124 自发性兴奋性突触后电流 

分 类 号：R742.1[医药卫生—神经病学与精神病学]