肝细胞肝癌组织异黏蛋白的表达变化及临床意义  

Expression and clinical significance of metadherin in hepatocellular carcinoma

作　　者：朱凯[1,2] 战昊 周俭[1,2] 樊嘉[1,2] 代智[1,2] 

机构地区：[1]复旦大学附属中山医院肝脏外科,复旦大学肝癌研究所,上海200032 [2]教育部癌变与侵袭原理重点实验室,上海200032

出　　处：《中国临床医学》2017年第3期334-338,共5页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金(81402376,81472218);复旦大学附属中山医院人才培养计划-优秀骨干计划(2015ZSYXGG)~~

摘　　要：目的:观察异黏蛋白(metadherin,MTDH)在肝细胞肝癌(HCC)组织中的表达变化,并探讨其可能的临床价值。方法:对含323例肝细胞肝癌患者肿瘤及癌旁正常肝脏组织的组织芯片进行免疫组化染色,分析MTDH的表达情况及其与肝癌患者临床特征之间的关系。进一步采用免疫共沉淀(Co-Immunoprecipitation,Co-IP)结合质谱(mass spectrometry)技术寻找异黏蛋白的功能复合体。结果:免疫组化结果显示MTDH在肝癌组织中表达明显高于癌旁正常肝脏组织,在有微血管侵犯、卫星结节、低分化和TNM分期Ⅱ~Ⅲ期的患者中升高尤其明显(P<0.01)。低MTDH表达组患者的1、3、5年生存率和无复发生存时间明显优于高MTDH表达组(P<0.05)。免疫共沉淀技术发现MTDH能与PRMT5形成蛋白复合体。结论:MTDH有助于判断肝癌患者术后预后,可作为潜在的预后指标。Objective：To investigate the expression of metadherin （MTDH） in hepatocellular carcinoma （HCC） and its role in promoting HCC metastasis.Methods：The expression of MTDH in HCC and adjucent normal tissues was detected.Co-Immunoprecipitation （Co-IP） and mass spectrometry was used to investigate the protein complex involving MTDH.Results：The expression of MTDH was significantly higher in HCC tumors than that in corresponding normal liver tissues, and tumors with microvascular invasion, pathological satellites, poor differentiation, or TNM stage Ⅱ-Ⅲ were prone to exhibit higher MTDH expression （P〈0.01）.The clinical outcome was poorer in the MTDH high group than that in the MTDH low group in both the 1-, 3-, 5-year overall survival rates （OS） and the 1-, 3-, 5-year cumulative recurrence rates （P〈0.05）.CO-IP results showed that MTDH can form a complex with PRMT5.Conclusions：MTDH can accurately predict the poor outcome of HCC patients after surgery, and is a great independent prognostic indicator.

关 键 词：异黏蛋白 肝细胞肝癌 肿瘤转移 预后 

分 类 号：R735.7[医药卫生—肿瘤]