多西他赛对乳腺癌细胞中Cav-1表达及细胞增殖的影响  

Effect and potential mechanism of Cav-1 in Docetaxel inhibiting breast cancer MCF-7 cell proliferation

作　　者：李颖[1] 尹良伟[2] 刘基巍[1] 刘鹏[2] 

机构地区：[1]大连医科大学附属第一医院肿瘤一科,辽宁大连116011 [2]大连医科大学附属大连市中心医院肿瘤内科三病房,辽宁大连116021

出　　处：《大连医科大学学报》2017年第4期318-322,共5页Journal of Dalian Medical University

基　　金：国家自然科学基金项目(81302310)

摘　　要：目的研究多西他赛对乳腺癌细胞中Cav-1(Cav-1)表达及细胞增殖的影响。方法通过Real-time PCR和Western-blot检测不同浓度多西他赛作用下乳腺癌细胞MCF-7中Cav-1的表达情况;野生型MCF-7细胞株为对照组,通过基因转染技术建立Cav-1过表达的MCF-7细胞株(转染组)及空载体的MCF-7细胞株(空载体组),利用CCK-8比色法分析多西他赛对各组细胞增殖的影响。通过Western-blot分别检测多西他赛作用下各组细胞中凋亡相关蛋白Bax和Bcl-2的表达情况以及PI3K/AKT信号转导通路的活化情况。结果在5~40μg/m L浓度范围内,随着多西他赛作用浓度的增大,MCF-7细胞中Cav-1的蛋白表达量逐渐增加。不同浓度多西他赛对MCF-7细胞的增殖均有明显的抑制作用;在同一浓度多西他赛作用下,转染Cav-1组细胞的增殖抑制率明显高于对照组和空载体组(P<0.01)。与对照组和空载体组相比,20μg/m L多西他赛作用后转染组细胞中Bax的表达升高(P<0.01),而Bcl-2的表达降低(P<0.01),并且磷酸化AKT(p-AKT)蛋白的表达水平下降(P<0.01),而对照组和空载体组之间Bax、Bcl-2以及p-AKT蛋白的表达则无明显差异(P>0.05)。结论多西他赛可以通过促进Cav-1的表达影响PI3K/AKT信号转导通路,进而调节凋亡相关蛋白的表达抑制MCF-7细胞的增殖。Objective To investigate the effect and potential mechanism of Cav - 1 （ Cav - 1 ） on the proliferation inhibiting of breast cancer MCF - 7 cell by Docetaxel. Methods The transfection group was established by stable transfection of pcDNA3.1 - Cav - 1 into MCF - 7 cells, while the vector group （ pcDNA3.1 vector transfected） and control group （ non - transfection MCF-7 cells） were used as control. The Cav- 1 expression of MCF-7 cells treated by Docetaxel was detec- ted by Real - time PCR and Western - blot. CCK - 8 assay was used to analyze the cell proliferation. The expression of Bax and Bcl - 2 proteins and the activation of PI3K/AKT signal pathway were examined by Western - blot. Results The expression of Cav - 1 in MCF - 7 cells was gradually enhanced with the increasing of Docetaxel concentration in the range of 5 - 40 μg/mL. The inhibitory rate of cell proliferation in Cav - 1 transfection group was significantly higher than that of the control groups after Docetaxel treatment （P 〈 0.01 ）. Compared with the control groups, Bax expression increased and Bcl -2 and p- AKT expression decreased in Cav- 1 transfection group （P 〈 0.01 ）, while there was no significant difference be- tween the two control groups （ P 〉 0.05 ）. Conslusion Docetaxel might inhibit the proliferation of MCF - 7 cells by promo- ting the Cav - 1 expression, which can regulate the apoptosis - related protein expression and suppress the activation of PI3K/AKT signaling pathway.

关 键 词：Cav-1 多西他赛 乳腺癌细胞 细胞增殖 基因治疗 

分 类 号：R737.9[医药卫生—肿瘤]