Galectin-3和上皮-间质转化相关标志物在卵巢癌中的表达及与淋巴结转移的关系  

Expression of galectin-3 and epithelial-mesenchymal transition-related markers in ovarian carcinoma and their correlation with lymph node metastasis

作　　者：黎晔[1] 吴共发[2] 林俊汕[2] 曾宇婷[2] 黄绮亭[2] 卢淮武[3] Li Ye Wu Gongfa Lin Junshan Zeng Yuting Huang Qitingz Lu Huaiwu(Department of Gynecology Department of Pathology, Zengcheng District People＇s Hospital of Guangzhou ＆ Zengcheng Branch of Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 511300 Department of Gynecologic Neoplasms, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 510120, China)

机构地区：[1]广州市增城区人民医院&中山大学孙逸仙纪念医院增城院区妇科,广州511300 [2]广州市增城区人民医院&中山大学孙逸仙纪念医院增城院区病理科,广州511300 [3]中山大学孙逸仙纪念医院妇瘤科,广州510120

出　　处：《中国组织化学与细胞化学杂志》2016年第6期517-522,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的研究卵巢癌中半乳糖凝集素-3(galectin-3)和上皮-间质转化(epithelial-mesenchymal transition,EMT)相关标记物的表达及与淋巴结转移之间的关系。方法免疫组织化学检测57例卵巢癌组织及13例淋巴结转移癌galectin-3和EMT标记物E-cadherin与vimenitn的表达,并分析其与临床病理的关系。结果 galectin-3在卵巢癌的阳性表达率为70.2%,其中Ⅲ+Ⅳ期的表达显著高于Ⅰ+Ⅱ期,有淋巴结转移者显著高于无淋巴结转移者;E-cadherin在卵巢癌的阳性表达率为75.4%,其中Ⅰ+Ⅱ期的表达显著高于Ⅲ+Ⅳ期,无淋巴结转移的表达显著高于有淋巴结转移者。Vimentin的阳性表达率为49.1%,其中Ⅲ+Ⅳ期的表达显著高于Ⅰ+Ⅱ期,高级别肿瘤的表达显著高于低级别,有淋巴结转移的表达显著高于无淋巴结转移者。Pearson相关分析显示,卵巢癌中galectin-3表达与E-cadherin表达呈负相关,而与vimentin呈正相关。结论卵巢癌中出现galectin-3表达和发生EMT的病例容易出现淋巴结转移,galectin-3和EMT标志物改变具有相关性,galectin-3可能通过EMT途径促进卵巢癌进展。Objective To study the expression of Galectin-3 and epithelial-mesenchymal transition （EMT） -related markers and their relationship with lymph node metastasis in epithelial ovarian carcinoma （ EOC）. Methods The expression of Galectin-3 and EMT- related markers E-cadherin and Vimentin was detected by immunohistochemistry in 57 samples of EOC including 13 cases of lymph node metastasis; their relationship with clinical pathology was analyzed. Results In EOC, the galectin-3 expression rate was 70. 2% ; it was significantly higher in Ⅲ＋ Ⅳ stages than in Ⅰ＋Ⅱ stages of tumor, and also higher in patients with than without lymph node metastasis. The positive rate of E-eadherin was 75.4% ; it was significantly higher in Ⅰ＋Ⅱ stages than in Ⅲ ＋ Ⅳ stages of tumor, and in patients without than with lymph node metastasis. The positive rate of Vimentin was 70. 2% ; it was significantly higher in Ⅲ＋Ⅳ stages than in Ⅰ＋Ⅱ stages of tumor, in high-grade than low-grade tumors, and in patients with than without lymph node metastasis. Pearson analysis showed that the expression of galectin-3 was negatively correlated with that of E-cadehrin while positively correlated with that of Vimentin. Conclusion Lymph node metastasis is prone to occur in EOC patients with galectin-3 expression and EMT. The expression of galectin-3 is correlated with that of EMT markers and may promote the progress of EOC through the EMT pathway.

关 键 词：卵巢癌 半乳糖凝集素-3 上皮-间质转化 

分 类 号：R737.31[医药卫生—肿瘤]